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Transgenic and Gene Knockout

A National Facility for Transgenic and Gene Knock-out Mice sponsored by the Department of Science and Technology (DST), Government of India has been set up at the CCMB with the following aims: 

  • Procurement and maintenance of transgenic and knock-out mouse models of human diseases where such animals are available elsewhere without restriction and provide facility and expertise to use the same in drug screening. 
  • Breeding of currently available transgenic and knock-out mouse models. 
  • Development of transgenic and gene knock-out technologies.
  • Development of new transgenic and gene knock-out mice models of human diseases. 

A modern mouse facility houses transgenic mice and other experimental mouse strains needed for creation of transgenic and gene knock-out mice. The mouse genetics laboratory includes well-furnished infrastructure for culture of embryonic stem cells, cryopreservation of embryos, micro-manipulation of embryos, animal surgery, construction of transgenes & gene knock-out vectors, identification of transgenic & knock-out mice, and analysis of gene expression.

To understand regulation of gene expression and to delineate the function of mammalian genes, we routinely create transgenic & gene knockout mice by performing DNA microinjection into mouse eggs, and genetic manipulation of mouse embryonic stem cells.