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Support
Facilities

Experimental
Farm, Horticulture and Landscaping
JONAKI,
the Labelled
Biomolecules Laboratory, is a unit of Board of
Radiation & Isotope Technology (BRIT),
Department of Atomic Energy. It is situated in the
campus of the
Centreer
for Cellular & Molecular Biology (CCMB). This
laboratory is a shining example of BRIT’s
commitment to provide labeled biomolecules and
different molecular biology kits to the
biotechnologists, molecular biologists and other
life science research scientists of the country.
Over a dozen 32P / 33P
labeled nucleotides and molecular biology kits are
supplied on a regular basis to various national
laboratories, universities and industries across
the country. Radioisotopes labeled nucleotides are
one of a few essential components of modern
research in biology, agriculture, healthcare,
medical sciences, biotechnology and genetic
engineering. 33P labeled nucleotide,
which is also a favoured tool for in situ
hybridization, DNA sequencing and DNA chip-
based gene expression studies, and is
considered as a bridge between radioisotopic and
non-isotopic labels, is currently synthesized in
this laboratory and supplied to various users.
Jonaki
had started a retail outlet for nuclear medicine
cold kits one year before to support the hospitals
in the city. It had also introduced PCR
Amplification kits, nucleotide mix and Taq DNA
polymerase to suit individual needs, priced
competitively and tested. Both have been a good
success.
It is constantly improvising its functioning to
maintain an excellent quality of its products and
its staff is constantly working in this direction.
All the 32P/33P nucleotides
are being supplied in very good purity, better
stability and specific activity. Its
molecular biology products like Taq DNA polymerase
and Molecular Biology kits are also being supplied
with good stability and very good performance. And
for this, Jonaki is rightly placed in the campus
of the
CCMB, which provides the infrastructure
facilities for its good functioning and suitable
scientific environment for its growth.
Jonaki has entered into a new business area, in
the field of molecular diagnostics through the
launch of its Gel based TB-PCR kit in August,
2008. It has also launched nucleic acid
purification kits based on silica membrane at
affordable prices to serve molecular biologists
and molecular diagnostics community across the
country without compromising with the quality. It
is preparing for extending its activities in the
field of DNA diagnostics and ELISA diagnostics.
Efforts are on for further improvisation. TB-PCR-ELISA
kit and Real Time TB-PCR kit are under clinical
trial and are
expected to be launched shortly. The ELISA
kit for the human thyroid hormone T3 is under
clinical trial and is also expected to be launched
shortly.
List of Products:
32P Nucleotides:
1. γ 32P ATP LCP 101 2. γ 32P
GTP LCP 1011
3. α 32P ATP LCP 106 4. α 32P
CTP LCP 109
5. α 32P GTP LCP 107 6. α 32P
UTP LCP 108
7. α 32P dATP LCP 103 8. α 32P
dCTP LCP 102
9. α 32P dGTP LCP 104
10.3′5′-a 32P pCP LCP 1010
11.32P-Orthophosphoric Acid LCP 32
The above products are available in two
formulations twice a month.
33P Nucleotides:
1. γ 33P ATP LCP 331
2. α 33P dATP LCP333 35S
Amino Acids:
1. 35S Methionine LCS 1/LCS 2
2. 35S Cystein LCS 3
3. 35S Methionine-Cysteine
Eleg mix LCS 7
4. 35S Glutathione LCS 6
5. Protein in
vivo twin label mix LCS 8
The following Molecular Biology kits are also
available.
CODE PRODUCT
LCK-1 Nick Translation Kit
LCK-2 Random Primer Kit
LCK-3 5′-End Labeling Kit
LCK-1301PCR Amplification Core Kit (100 reaction
kit)
LCK-1302PCR Amplification Core Kit (50 reaction
kit)
LCK-1303PCR Amplification Core Kit (25 reaction
kit)
LCK-1401PCR Amplification Core Kit (100 reaction
kit)
LCK-1402PCR Amplification Core Kit (50 reaction
kit)
LCK-1403PCR Amplification Core Kit (25 reaction
kit)
LCK-1501 PCR DNA Labelling Kit (50 reaction kit)
LCK-1502
PCR DNA Labelling Kit (25 reaction kit)
LCK-1601 dNTP mix for PCR (1 set of 4 dNTPs in 4 x
25 μl)
LCK-1602 dNTP mix for PCR (3 sets
of 4 dNTPs in 4 x 25 μl)
LCK-1603 dNTP mix for PCR (5 sets
of 4 dNTPs in 4 x 25 μl)
LCK-1604 dNTP mix for PCR (10 sets
of 4 dNTPs in 4 x 25 μl)
LCE-101 Taq DNA Polymerase Enzyme (100 Units)
LCE-102 Taq DNA Polymerase Enzyme (250 Units)
LCE-103 Taq DNA Polymerase Enzyme (1000 Units)
LCE-104 Taq DNA Polymerase Enzyme (2000 – 5000
Units)
LCE-105 Taq DNA Polymerase Enzyme (7000 Units)
New Products Introduced:
CODE PRODUCT
LCK1701 M.tuberculosis PCR detection kit (25
reaction kit)
LCK 1702M.tuberculosis PCR detection kit (50
reaction kit)
LCK 18 DNA Isolation kit (Blood) (50 reaction kit)
LCK 19 DNA Isolation kit (Blood) (100 reaction
kit)
LCK 20 DNA Isolation kit (Bacteria) (50 reaction
kit)
LCK 21 DNA Isolation kit (Bacteria) (100 reaction
kit)
LCK 22 DNA Isolation kit (Plasmid) (50 reaction
kit)
LCK 23 DNA Isolation kit (Plasmid) (100 reaction
kit)
LCK 24 DNA Gel Purification kit (50 reaction kit)
LCK 25 DNA Gel Purification kit (100 reaction kit)
LCK 26 PCR Product Purification kit (50 reaction
kit)
LCK 27 PCR Product Purification kit (100 reaction
kit)
Cold kits for formulation of
99mTcradiopharmaceuticals
CODE PRODUCT
TCK-5 Kit for Sulphur colloid injection
Use: For scintigraphy of the
reticuloendothelial system
TCK-7 Kit for DTPA injection
Use: For renal function studies and brain
scintigraphy
TCK-15 Kit for GHA injection
Use: For renal scintigraphy
TCK-16 Kit for Phytate injection
Use: For scintigraphy of liver
TCK-30 Kit for MDP injection
Use: For bone scintigraphy
TCK-35 Kit for DMSA injection
Use: Fjor
renal and tumour scintigraphy
TCK-38 Kit for Sn-Pyp injection
Use: For in
vivo labeling of RBC
TCK-39 Kit for Mebrofenin injection
Use: For hepatobiliary scintigraphy
TCK-42 Kit for ECD injection
Use: For brain perfusion imaging
TCK-43 Kit for EC injection
Use: For renal dynamic function studies
TCK-50 Kit for MIBI injection
Use: For myocardial perfusion imaging
Products to be introduced shortly
1) Real time TB-PCR kit
2) TB-PCR-ELISA kit
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