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Reference Panels for identification and individualization of Species and Varieties
| DNA typing of Mulberry genome was undertaken using multiple DNA marker techniques like RAPD, Flourescence-AFLP and Flourescence-microsatellites (SSRs) for their genetic characterization and also to create genotype-specific diagnostic patterns. The DNA profiles, thus obtained, were analyzed to develop Reference DNA Polymorphism data panels that can be used readily to generate "Molecular ID's" unique to specific Mulberry genotypes. Efforts were made to identify sets of microsatellite markers (which provide more robust easily revisitable signatures compared to RAPDs/AFLPs etc.) and that were relatively more polymorphic for easy individualization of Species and Varieties. Such reference polymorphism panels are expected to be of immense use for genotype registration, IPR protection and for other genetic studies. |
| Two of the representative Polymorphism Data Panels for with some of the microsatellite markers are shown below. Information /data for other markers can be obtained from CCMB on specific requests on the contact address given below. |
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AFLP
fingerprints as 'Molecular ID's - Primer Combination1:E-ACG/M-CAA(Species)
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AFLP
fingerprints as 'Molecular ID's - Primer Combination1:E-ACG/M-CAA(Varieties)
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Conceptualisation and Co-ordination: Dr. Ramesh Aggarwal Design/development: D. Udaykumar and Dr. R. Rajkumar |
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