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CSIR - Centre for Cellular & Molecular Biology

Council of Scientific and Industrial Research
Ministry of Science & Technology, Govt. of India

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Swasti Raychaudhuri

Principal Scientist
Email: rcswasti@ccmb.res.in
Phone: +91-040-27192558

Research Interests

Toxic and beneficial outcome of protein aggregation inside cells

With the increasing life expectancy, developing effective treatments for numerous degenerative, age-related diseases becomes of enormous medical, social and economic relevance. Hence, research on cellular and organismal aging is now the focus of several leading laboratories and has provided different hypotheses about the causes of aging. One prominent hypothesis suggests that a gradual, age-dependent decline of protein homeostasis (proteostasis) accompanied by increasing protein-aggregation is the underlying cause of many age-related loss-of-function diseases. In our lab we perform systemic analysis of protein-aggregation in age-related proteostasis stress models. We are interested to identify the response of the cellular proteome against the newly triggered aggregates due to various stresses. Simultaneously, we investigate how components of the proteostasis network, including molecular chaperones, degradation machinery and others collaborate to maintain the integrity of the proteome in the face of protein-aggregation stresses. The specific questions include:

  • Proteostatic control on protein-complex biogenesis!!
  • Cellular response to protein aggregation!!

We use modern cell biology and state-of-the-art proteomics arsenal to address our questions.

Selected Publications

Increased supraorganization of respiratory complexes is a dynamic multistep remodelling in response to proteostasis stress. Rawat S, Ghosh S, Mondal D, Anusha V, Raychaudhuri S. J Cell Sci. 2020;133(18):jcs248492. doi: 10.1242/jcs.248492.
Read the interview of the first author, Shivali Rawat here

Aggregation of Respiratory Complex Subunits Marks the Onset of Proteotoxicity in Proteasome Inhibited Cells. Rawat S, Anusha V, Jha M, Sreedurgalakshmi K, Raychaudhuri S. J Mol Biol. 2019 431(5):996-1015. 

Interplay of Acetyltransferase EP300 and the Proteasome System in Regulating Heat Shock Transcription Factor 1. Raychaudhuri S, Loew C, Koerner R, Pinkert S, Theis M, Hayer-Hartl M, Buchholz F, Hartl FU. Cell. 2014 Feb 27;156(5):975-85. 

Firefly luciferase mutants as sensors of proteome stress.Gupta R, Kasturi P, Bracher A, Loew C, Zheng M, Villella A, Garza D, Hartl FU, Raychaudhuri S.Nature Methods. 2011 Sep 4; 8(10):879-84. 

HYPK, a Huntingtin interacting protein, reduces aggregates and apoptosis induced by N-terminal Huntingtin with 40 glutamines in Neuro2a cells and exhibits chaperone-like activity.Raychaudhuri S, Sinha M, Mukhopadhyay D, Bhattacharyya NP. Hum Mol Genet. 2008 Jan 15;17(2):240-55. 

Education & Experiance

Publications

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Swasti Raychaudhuri

Principal Scientist

2558

rcswasti@ccmb.res.in

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Shivali Rawat

Senior Research Fellow

2551

shivali@ccmb.res.in

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Shemin Mansuri

Senior Research Fellow

2551

shemin.16@ccmb.res.in

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Harshit Vaish

Junior Research Fellow

2551

harshit@ccmb.res.in

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Pooja Gupta

Junior Research Fellow

2556

poojagupta@ccmb.res.in

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Suparna Ghosh

Junior Research Fellow

2552

suparna@ccmb.res.in

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Richa Singh

Junior Research Fellow (Project)

2551

richa@ccmb.res.in

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Durgempudi Varsha Reddy

Junior Research Fellow (Project)

2551

vasha-reddy@ccmb.res.in