A National Facility for Transgenic and Gene Knock-out Mice sponsored by the Department of Science and Technology (DST), Government of India has been set up at the CCMB with the following aims:
Procurement and maintenance of transgenic and knock-out mouse models of human diseases where such animals are available elsewhere without restriction and provide facility and expertise to use the same in drug screening.
Breeding of currently available transgenic and knock-out mouse models.
Development of transgenic and gene knock-out technologies.
Development of new transgenic and gene knock-out mice models of human diseases.
A modern mouse facility houses
transgenic mice and other experimental mouse strains needed for creation of
transgenic and gene knock-out mice. The mouse genetics laboratory includes
well-furnished infrastructure for culture of embryonic stem cells, cryopreservation
of embryos, micro-manipulation of embryos, animal surgery, construction of
transgenes & gene knock-out vectors, identification of transgenic &
knock-out mice, and analysis of gene expression.
To understand regulation of gene expression and to delineate the
function of mammalian genes, we routinely create transgenic & gene knockout
mice by performing DNA microinjection into mouse eggs, and genetic manipulation
of mouse embryonic stem cells.