First slide
Research Interests

Early diagnosis of infections, genetic disorders, and metabolic disorders are some of the most serious challenges to the health care system worldwide, especially in India, and are essential in the management of infection/disease, treatment decisions, and response to treatment. The current diagnostic tools include; (1) serological assays, (2) molecular assays, and (3) routine biochemical tests. All these methods lack accuracy, require trained technicians, are expensive as well as time-consuming. In my group, we are involved in developing diagnostic tools that are “affordable”; for example developing microfluidic paper-based analytical devices (µPAD) or paper-microfluidic devices coupled with an instrument-free or portable device, which is coupled with ambient temperature amplification technology while using optical and/or electrochemical detection system for quantification of nucleic acid (NA). Apart from this, my group is also interested in developing: 

Paper-based devices as Raman immune--sensor.

  • Non-conventional methods to fabricate microfluidic devices.
  • Biopolymer microfluidic devices for tissue engineering and cell culture.
  • 3D printing and 3D cell culture.
  • Generation of "site targeted" drug delivery vectors for drug delivery and diagnosis, using microfluidic devices.

Selected Publications

An affordable, rapid determination of total lipid profile using a paper-based microfluidic device, ShahilaParween, P. Debishree Subudhi, Amit Asthana, Sensors & Actuators: B. Chemical, 2019, 285, 405-412.

Fabrication of cost-effective and efficient paper-based device for viscosity measurement, Anirudh Rayaprolu; Saurabh K Srivastava; LavleenBhati; Ketan Anand; Amit Asthana, Ph.D.; Ch. Mohan Rao, Analytica Chimica Acta, 2018, 1044,86-92.

Chitosan as a biomaterial in drug delivery and tissue engineering, S. M. Ahsan. M. Thomas, K. K. Reddy, S. G. Sooraparaju, A. Asthana, I. Bhatnagar, Int. J. Biol. Macromol., 2018, 110, 97-109.

Chitosan stabilized gold nanoparticle-mediated self-assembled gliPnanobiosensor for diagnosis of Invasive Aspergillosis, I. Bhatnagar, K. Mahato, K. K. Reddy Ealla, A. Asthana, P. Chandra, Int. J. Biol. Macromol., 2018, 110, 449-456.

Foil assisted replica molding for the fabrication of microfluidic devices and their application in vitro, Issac J. Micheal, Aditya J. Vidyasagar, Kiran Kumar Bokara, Naveen Kumar Mekala, Amit Asthana and Ch Mohan Rao, Lab-on-a-chip,2014, 14 (19), 3695 -3699.

Education & Experiance

P.G: Barkatullah University, Bhopal ; Applied Chemistry ; 1995
Ph.D: Detection Enhancement and Separation of Toxic Compounds Using Capillary Electrophoresis and Other Nano & Micro- Scale Analytical Techniques ; Barkatullah University, Bhopal ; 2000
Post.Doc:

Microfluidics, Biopolymer devices ; Brainpool Fellowship (K. S. F.), Chugnam National University, South Korea ; 2006-2008

Meso and Micro-porous materials ; Research Professor, Chugnam National University, South Korea ; 2004-2005

Micro-fuel cells ; DARPA post-doctoral fellow, the University of Illinois at Urbana-Champaign, USA ; 2001-2003

Microfluidics, separation of large DNA molecules ; Curie Post-doctoral Fellow, Institute Curie, France ; 2000-2001

Experience:

Ramalingaswami Fellow, Centre for Cellular and Molecular Hyderabad, India; 2011-2013

Senior Professional Officer, Australian Nationa Fabrication Facility (QLD-Node), Australia, 2008-11

Assistant Professor, Ambala College of Engineering and Applied Research, India, 2005-2006

Assistant Professor, Bio-Engineering Research Center, Rai Foundation College, Faridabad, India 2004-2005

CSIR Quick Hire Fellow (R4), Advanced Materials & Processes Research Institute, India, 2003-2004

Senior Research Fellow, Advanced Materials & Processes Research Institute, India, 1998-2000

Visiting Fellow, University of Amsterdam, The Netherlands, 1997-1998

Junior Research Fellow Advanced Materials & Processes Research Institute, India, 1998-2000

Patents

InventionCountries of filing the patentApplication numberPublication numberStatusGrant Number
A rapid, low cost process for the preparation of SERS substrate and SERS substrate prepared thereby India 201811023895 201811023895 Published Pending
A rapid, low cost process for the preparation of SERS substrate and SERS substrate prepared thereby PCT International PCT/IN2019/050102 WO/2020/003321 Published Pending
A novel facile aqueous-based extraction of progesterone metabolites from faeces sample for non-invasive, simple, affordable and farmer-friendly paper-based kit for pregnancy detection in cattle and buffaloes India 201911008655 Filed Pending
A novel facile aqueous-based extraction of progesterone metabolites from faeces sample for non-invasive, simple, affordable and farmer-friendly paper-based kit for pregnancy detection in cattle and buffaloes PCT International PCT/IN2020/050202 Filed Pending

Publications

TitleJournalYear
An affordable, rapid determination of total lipid profile using a paper-based microfluidic device Sensors & Actuators: B. Chemical 2019
Chitosan stabilized gold nanoparticle mediated self-assembled gliP nanobiosensor for diagnosis of Invasive Aspergillosis Int. J. Biol. Macromol 2018
Chitosan as biomaterial in drug delivery and tissue engineering Int. J. Biol. Macromol 2018
Fabrication of cost-effective and efficient paper-based device for viscosity measurement Analytica Chimica Acta 2018
A microfluidic device approach to generate hollow alginate microfibers with controlled wall thickness and inner diameter Journal of Applied Physics 2015
Foil assisted replica molding for fabrication of microfluidic devices and their application in vitro Lab-on-a-Chip 2014
Droplet based microfluidics (Book Chapter) in Methods in Molecular Biology: Microfluidic Diagnostics, G. Jenkins and C. Mansfield, eds., Humana Press 2013
Rapid and cost-effective fabrication of selectively permeable calcium-alginate microfluidic device using modified embedded template method Biomicrofluidics 2012
Fabrication and Characterization of Gold Nanohole Electrode Ensemble Sensors & Actuators: B Chemical 2012
Bromo-oxidation reaction in enzyme loaded alginate hollow microfiber fabricated using microfluidic device Biomicrofluidics 2011
Bromo-oxidation reaction in enzyme loaded alginate hollow microfiber fabricated using microfluidic device Biomicrofluidics 2011
Fabrication of Multilayer Microstructure using Dry Film Resist and Deep Reactive Ion Etcher Micro & Nano Letters 2010
A facile single step fabrication of microchannels with varying sizes Lab-on-a-chip 2009
A continuous-exchange cell-free protein synthesis system fabricated on a chip Analytical Biochemistry 2007
Novel transparent polysilazane derived solvent resistant, biocompatible microchannels and substrates: application in micro system technology Lab-on-a-Chip 2006
Fabrication of meso and microporous ceramic microchannels Journal of Materials Research 2006
Fabrication of ceramic microchannels with tailored pores Materials Science Forum 2006
DNA mutation analysis based on capillary electrochromatography using colloidal poly (n-isopropylacrylamide) particles as pseudostationary phase Talanta 2006
Rapid determination of sulfonamides in meat and milk by micellar electrokinetic chromatography with fluorescence detection Analytica Chimica Acta 2005
Fast separation and sensitive detection of carcinogenic aromatic amines by reversed phase liquid chromatography coupled with electro-chemical detector Journal of Chromatography "A" 2005
Fabrication and characterization of a silicon-based millimeter scale, microfabricated PEM fuel cell operating with gaseous hydrogen, methanol, and formic acid as fuels Sensors and Actuators-B: Chemical 2005

Amit Asthana

Principal Scientist

5547

aa@ccmb.res.in

Ira Bhatnagar

Principal Scientist

5534

ira@ccmb.res.in