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Research Interests

Understanding the quiescence program and its benefits for stem cell function.Most cells in adult tissues have permanently ceased proliferation. However, stem cells in regenerating tissues must attain and maintain a temporary state of quiescence so that a reserve pool is available for future unpredictable bouts of repair. The programs that control the cell cycle and differentiation are coordinated to ensure the correct balance of stem cells and differentiated cells in regenerating tissue, but the mechanisms that direct cells into reversible vs. irreversible arrest are poorly understood.

Selected Publications

Sachidanandan, C., Ramkumar, S. and Dhawan, J. (2002) Tristetraprolin and LPS-inducible CXC chemokine are transiently induced in presumptive satellite cells in response to skeletal muscle injury. J. Cell. Sci. 115 (13) 2701-2712.

Dhawan, J. and Helfman, D.M. (2004) Modulation of acto-myosin contractility in skeletal muscle myoblasts uncouples growth arrest from differentiation J Cell Sci 117: 3735-3748.

Dhawan, J. and Rando, T.A. (2005). Stem Cells in Postnatal Myogenesis: Molecular Mechanisms of Satellite Cell Quiescence, Activation and Replenishment. Trends Cell Biol. 15:666-676.

Sebastian, S., Sreenivas, P., Ramkumar, S., Prashanth, K., Cheedipudi,S., Pavlath G.K. and Dhawan, J. (2009). MLL5, a trithorax homolog, indirectly regulates H3K4 methylation, represses Cyclin A2, expression, and promotes myogenic differentiation. Proc. Natl. Acad. Sci. USA. 106: 4719-4724.

Sambasivan, R., Cheedipudi, S., Pasupuleti, N., Saleh, A., Pavlath, G.K. and Dhawan, J. (2009). The small chromatin protein p8/Nupr1 co-ordinates anti-proliferative and pro-myogenic proteins targeting the Myogenin promoter J. Cell Sci. 122: 348.

Education & Experiance

P.G: Botany ; Delhi University ; 1983
Ph.D: Adhesion-dependent gene regulation in mouse fibroblasts and myoblasts ; Boston University ; 1991
Post.Doc:

Myoblast-mediated gene therapy ; Stanford University, Dept of Molecular Pharmacology ; 1990-95

Muscular dystrophy ; Dept of Neurology and Neurological Sciences ; 1995-96

Experience:

Senior Professor and Dean, Institute for Stem Cell Science and Regenerative Medicine (InStem) 2009-2014 Visiting Professor, InStem 2014-to date

Publications

TitleJournalYear
Cytoplasmic sequestration of the RhoA effector mDiaphanous1 by Prohibitin2 promotes muscle differentiation Scientific Reports (accepted) 2019
Cell density overrides the effect of substrate stiffness on human mesenchymal stem cells’ morphology and proliferation Biomaterials Science, 6(5): 1109–1119. doi:10.1039/C7BM00853H 2018
Induction of quiescence (G0) in bone marrow stromal stem cells enhances their stem cell characteristics Stem Cell Research, 30: 69-80. doi: 10.1016/j.scr.2018.05.010 2018
Cycling to Meet Fate: Connecting pluripotency to the cell cycle Frontiers in Cell and Developmental Biology, 6: 57. doi: 10.3389/fcell.2018.00057 2018
The transcription factor Lef1 switches partners from ?-catenin to Smad3 during muscle stem cell quiescence Sci. Signal. 24 Jul 2018:Vol. 11, Issue 540, eaan3000 DOI: 10.1126/scisignal.aan3000 Abstract | Full Text 2018
Mimicking muscle stem cell quiescence in culture: methods for synchronization in reversible arrest Muscle Stem Cells: Methods and Protocols, 1556:283-302. doi: 10.1007/978-1-4939-6771-1_15 2017
C-State: An interactive web app for simultaneous multi-gene visualization and comparative epigenetic pattern search BMC Bioinformatics, 18 (Suppl 10):392. doi: 10.1186/s12859-017-1786-6 2017
Low oxygen tension enhances expression of myogenic genes when human myoblasts are activated from G0 arrest PLOS One, 11:e0158860. doi: 10.1371/journal.pone.0158860 2016
Exosomes: mobile platforms for targeted and synergistic signaling across cell boundaries Cellular and Molecular Life Sciences, 74 (9): 1567-76 doi:10.1007/s00018-016-2413-9 2016
Distinguishing states of arrest: Genome-wide descriptions of cellular quiescence using ChIP-seq and RNA-seq analysis Methods in Molecular Biology, 1686:215-239. doi: 10.1007/978-1-4939-7371-2_16 2016
Quiescence in Adult Stem Cells: Biological Significance and Relevance to Tissue Regeneration Stem Cells, 33(10):2903-12. doi: 10.1002/stem.2056 2015
A fine balance: Epigenetic control of cellular quiescence by the tumor suppressor PRDM2/RIZ at a bivalent domain in the Cyclin A gene Nucleic Acids Research, 43(13):6236-56. doi: 10.1093/nar/gkv567 2015
Epigenetic mechanisms and boundaries in the regulation of mammalian Hox clusters Mechanisms of Development, 138 Pt 2:160-169. doi: 10.1016/j.mod.2015.07.015 2015
Identification of PRDM2 regulated genes in quiescent C2C12 myoblasts Genomics Data, 6:264-266. doi: 10.1016/j.gdata.2015.10.004 2015
Decoding the stem cell quiescence cycle: lessons from yeast for regenerative biology Journal of Cell Science, 128, 4467-4474 doi:10.1242/jcs.177758 2015
A ChIP-on-chip tiling array approach detects functional histone-free regions associated with boundaries at vertebrate HOX genes Genomics Data, 2:78-81. doi: 10.1016/j.gdata.2014.05.001 2014
Role of actin filaments in correlating nuclear shape and cell spreading PLOS One, 9(9):e107895. doi: 10.1371/journal.pone.0107895 2014
Vertebrate Hedgehog is secreted on two types of extracellular vesicles with different signaling properties Scientific Reports, 4:7357. doi: 10.1038/srep07357 2014
High-wire act: The poised genome and cellular memory FEBSJ, 282(9):1675-91. doi: 10.1111/febs.13165 2014
Deep sequencing reveals unique small RNA repertoire that is regulated during head regeneration in Hydra magnipapillata Nucleic Acids Research, 41(1):599-616. doi: 10.1093/nar/gks1020 2013
Applications of Microfabrication and Microfluidic Techniques in Mesenchymal Stem Cell Research Microfluidic Technologies for Human Health, 69-95. doi: 10.1142/9789814405522_0004 2013
Vertebrate GAGA factor associated insulator elements demarcate homeotic genes in the HOX clusters Epigenetics & Chromatin, 6(1):8. doi: 10.1186/1756-8935-6-8 2013
A novel in vitro model for studying quiescence and activation of primary isolated human myoblasts PLOS One, 8(5):e64067. doi: 10.1371/journal.pone.0064067 2013
Distinct transcriptional networks in quiescent myoblasts: a role for Wnt signaling in reversible vs. irreversible arrest PLOS One, 8(6):e65097. doi: 10.1371/journal.pone.0065097 2013
Regulation of cellular chromatin state: insights from quiescence and differentiation Organogenesis, 6(1):37-47 2010
The paternal hidden agenda: Epigenetic inheritance through sperm chromatin Epigenetics, 5(5):386-91 2010
MLL5, a trithorax homolog, indirectly regulates H3K4 methylation, represses Cyclin A2, expression, and promotes myogenic differentiation PNAS, 106(12):4719-24. doi: 10.1073/pnas.0807136106 2009
The small chromatin protein p8/Nupr1 co-ordinates anti-proliferative and pro-myogenic proteins targeting the Myogenin promoter Journal of Cell Science, 122: 3481-3491; doi: 10.1242/jcs.048678 2009
Consensus Guidance for Banking and Supply of Human Embryonic Stem Cell Lines for Research Purposes - International Cell Banking Initiative Stem Cell Reviews and Reports, 5(4):301-14. doi: 10.1007/s12015-009-9085-x 2009
A gene trap strategy identifies quiescence induced genes in synchronized myoblasts Journal of Biosciences, 33(1):27-44 2008
The RhoA effector mDiaphanous regulates MyoD expression and cell cycle progression via SRF-dependent and SRF-independent pathways Journal of Cell Science, 120 (Pt 17):3086-98. doi: 10.1242/jcs.006619 2007
Modulation of AP and DV signalling pathways by the homeotic gene Ultrabithorax during haltere development in Drosophila Developmental Biology, 291(2):356-67, doi: 10.1016/j.ydbio.2005.12.022 2006
Stem Cells in Postnatal Myogenesis: Molecular Mechanisms of Satellite Cell Quiescence, Activation and Replenishment Trends in Cell Biology, 15(12):666-73, doi: 10.1016/j.tcb.2005.10.007 2005
Modulation of acto-myosin contractility in skeletal muscle myoblasts uncouples growth arrest from differentiation Journal of Cell Science, 117(Pt 17):3735-48. doi: 10.1242/jcs.01197 2004
Skeletal Muscle Progenitor Cells in Development and Regeneration Proc. Indian Natn. Sci. Acad, B69 (5) 719-740 2003
Tristetraprolin and LPS-inducible CXC chemokine are transiently induced in presumptive satellite cells in response to skeletal muscle injury Journal of Cell Science, 115 (13) 2701-2712 2002
Distinct changes in intranuclear lamin A/C organization during myoblast differentiation. Journal of Cell Science, 114: 4001-4011 2001

Jyotsna Dhawan

Emeritus Scientist

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jdhawan@ccmb.res.in

Puja Singh

DST-Inspire Faculty

puja.singh@ccmb.res.in

Prabhavathy Devan

Research Associate (DBT)

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Gunjan Purohit

Research Associate (Project)

0

Lamuk Zaveri

PDF (Project)

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Sujoy Deb

Senior Research Fellow

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sujoy@ccmb.res.in

Debrya Saha

Senior Research Fellow

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Swetha S

Junior Research Fellow

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Priti A S

Junior Research Fellow

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Ananga Ghosh

Project Assistant-II

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