Training on Faster COVID-19 Testing
CSIR - Centre for Cellular & Molecular Biology
Council of Scientific and Industrial Research
Ministry of Science & Technology, Govt. of India
Emeritus Scientist
Email: jdhawan@ccmb.res.in
Phone: +91-040-27192544
Understanding the quiescence program and its benefits for stem cell function.Most cells in adult tissues have permanently ceased proliferation. However, stem cells in regenerating tissues must attain and maintain a temporary state of quiescence so that a reserve pool is available for future unpredictable bouts of repair. The programs that control the cell cycle and differentiation are coordinated to ensure the correct balance of stem cells and differentiated cells in regenerating tissue, but the mechanisms that direct cells into reversible vs. irreversible arrest are poorly understood.
Selected Publications
Sachidanandan, C., Ramkumar, S. and Dhawan, J. (2002) Tristetraprolin and LPS-inducible CXC chemokine are transiently induced in presumptive satellite cells in response to skeletal muscle injury. J. Cell. Sci. 115 (13) 2701-2712.
Sebastian, S., Sreenivas, P., Sambasivan, R., Prashanth, K., Cheedipudi,S., Pavlath G.K. and Dhawan, J. (2009). MLL5, a trithorax homolog, indirectly regulates H3K4 methylation, represses Cyclin A2, expression, and promotes myogenic differentiation. Proc. Natl. Acad. Sci. USA. 106: 4719-4724.
Cheedipudi, S*, Puri DR*, Saleh, A, Gala, HP, Rumman, M, Pillai, MS, Sreenivas, P. Arora, R, Sellathurai, J, Schroeder, HD, Mishra, RK and Dhawan, J. (2015). A fine balance: Epigenetic control of cellular quiescence by the tumor suppressor PRDM2/RIZ at a bivalent domain in the Cyclin A gene. Nucleic Acids Res. Jun 3. pii: gkv567. [Epub ahead of print]. *equal contribution
Aloysius A, DasGupta R and Dhawan J (2018) Lef1 switches partners from ?-catenin to Smad3 for transcriptional activation of Tcf/Lef1 target genes in quiescent muscle stem cells Science Signaling 11, 540, eaan3000. DOI: 10.1126/scisignal.aan3000
Venugopal N, Ghosh A, Gala HP, Aloysius A, Vyas N and J Dhawan (2020) The primary cilium dampens proliferative signaling and represses a G2/M transcriptional network in quiescent myoblasts BMC Cell Mol Biol doi.org/10.1186/s12860-020-00266-1.
Hardik P. Gala*, Debarya Saha*, Nisha Venugopal, Ajoy Aloysius, Gunjan Purohit, Jyotsna Dhawan (2022) A transcriptionally repressed quiescence program is associated with paused RNAPII and is poised for cell cycle reentry J Cell Sci 135 (15): jcs259789. https://doi.org/10.1242/jcs.259789 *Equal contribution
Education & Experience
| P.G: | Botany ; Delhi University ; 1983 |
| Ph.D: | Adhesion-dependent gene regulation in mouse fibroblasts and myoblasts ; Boston University ; 1991 |
| Post.Doc: | Myoblast-mediated gene therapy ; Stanford University, Dept of Molecular Pharmacology ; 1990-95 Muscular dystrophy ; Dept of Neurology and Neurological Sciences ; 1995-96 |
| Experience: | Scientist G and Group Leader CCMB (till Mar 2019); |
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| Title | Journal | Year |
|---|---|---|
| Opinion: The cilium-centrosome axis in coupling cell cycle exit and cell fate | J. Cell Sci. doi:10.1242/jcs.260454 | 2023 |
| Editorial: Cellular dormancy—State determination and plasticity | Front. Cell Dev. Biol. 10:984347. | 2022 |
| PTPRU, a quiescence-induced receptor tyrosine phosphatase negatively regulates osteogenic differentiation of human mesenchymal stem cells | BBRC https://doi.org/10.1016/j.bbrc.2022.10.062 | 2022 |
| Editorial: Cellular dormancy-State determination and plasticity. | Front. Cell Dev. Biol. 10:984347 | 2022 |
| A transcriptionally repressed quiescence program is associated with paused RNAPII and is poised for cell cycle reentry J | J Cell Sci 135 (15): jcs259789.
https://doi.org/10.1242/jcs.259789 |
2022 |
| mRNP granule proteins Fmrp and Dcp1a differentially regulate mRNP complexes to contribute to control of muscle stem cell quiescence and activation. | BMC Skeletal Muscle 11:18 https://doi.org/10.1186/s13395-021-00270-9 |
2021 |
| Inducible expression of Oct-3/4 reveals synergy with Klf4 in targeting Cyclin A2 to enhance proliferation during early reprogramming | BBRC doi: https://doi.org/10.1101/2021.03.16.435687 | 2021 |
| The primary cilium dampens proliferative signaling and represses a G2/M transcriptional network in quiescent myoblasts | BMC Cell Mol Biol doi.org/10.1186/s12860-020-00266-1. | 2020 |
| Comparative nuclear matrix proteome analysis of skeletal muscle cells in different cellular states | Cell Biol Int. 2020;1-19. DOI: 10.1002/cbin.11499 | 2020 |
| Adult muscle stem cells: Exploring the links between systemic and cellular metabolism | Frontiers in Cell Dev Bio, DOI: 10.3389/fcell.2019.00312 |
2019 |
| Cytoplasmic sequestration of the RhoA effector mDiaphanous1 by Prohibitin2 promotes muscle differentiation | Sci. Rep. (2019) 9:8302 doi.org/10.1038/s41598-019-44749-4 |
2019 |
| Cell density overrides the effect of substrate stiffness on human mesenchymal stem cells | Biomaterials Science, 6(5): 1109–1119. doi:10.1039/C7BM00853H | 2018 |
| Induction of quiescence (G0) in bone marrow stromal stem cells enhances their stem cell characteristics | Stem Cell Research, 30: 69-80. doi: 10.1016/j.scr.2018.05.010 | 2018 |
| Cycling to Meet Fate: Connecting pluripotency to the cell cycle | Frontiers in Cell and Developmental Biology, 6: 57. doi: 10.3389/fcell.2018.00057 | 2018 |
| The transcription factor Lef1 switches partners from ?-catenin to Smad3 during muscle stem cell quiescence | Sci. Signal. 24 Jul 2018:Vol. 11, Issue 540, eaan3000 DOI: 10.1126/scisignal.aan3000 Abstract | Full Text | 2018 |
| Mimicking muscle stem cell quiescence in culture: methods for synchronization in reversible arrest | Muscle Stem Cells: Methods and Protocols, 1556:283-302. doi: 10.1007/978-1-4939-6771-1_15 | 2017 |
| C-State: An interactive web app for simultaneous multi-gene visualization and comparative epigenetic pattern search | BMC Bioinformatics, 18 (Suppl 10):392. doi: 10.1186/s12859-017-1786-6 | 2017 |
| Low oxygen tension enhances expression of myogenic genes when human myoblasts are activated from G0 arrest | PLOS One, 11:e0158860. doi: 10.1371/journal.pone.0158860 | 2016 |
| Exosomes: mobile platforms for targeted and synergistic signaling across cell boundaries | Cellular and Molecular Life Sciences, 74 (9): 1567-76 doi:10.1007/s00018-016-2413-9 | 2016 |
| Distinguishing states of arrest: Genome-wide descriptions of cellular quiescence using ChIP-seq and RNA-seq analysis | Methods in Molecular Biology, 1686:215-239. doi: 10.1007/978-1-4939-7371-2_16 | 2016 |
| Quiescence in Adult Stem Cells: Biological Significance and Relevance to Tissue Regeneration | Stem Cells, 33(10):2903-12. doi: 10.1002/stem.2056 | 2015 |
| A fine balance: Epigenetic control of cellular quiescence by the tumor suppressor PRDM2/RIZ at a bivalent domain in the Cyclin A gene | Nucleic Acids Research, 43(13):6236-56. doi: 10.1093/nar/gkv567 | 2015 |
| Epigenetic mechanisms and boundaries in the regulation of mammalian Hox clusters | Mechanisms of Development, 138 Pt 2:160-169. doi: 10.1016/j.mod.2015.07.015 | 2015 |
| Identification of PRDM2 regulated genes in quiescent C2C12 myoblasts | Genomics Data, 6:264-266. doi: 10.1016/j.gdata.2015.10.004 | 2015 |
| Decoding the stem cell quiescence cycle: lessons from yeast for regenerative biology | Journal of Cell Science, 128, 4467-4474 doi:10.1242/jcs.177758 | 2015 |
| A ChIP-on-chip tiling array approach detects functional histone-free regions associated with boundaries at vertebrate HOX genes | Genomics Data, 2:78-81. doi: 10.1016/j.gdata.2014.05.001 | 2014 |
| Role of actin filaments in correlating nuclear shape and cell spreading | PLOS One, 9(9):e107895. doi: 10.1371/journal.pone.0107895 | 2014 |
| Vertebrate Hedgehog is secreted on two types of extracellular vesicles with different signaling properties | Scientific Reports, 4:7357. doi: 10.1038/srep07357 | 2014 |
| High-wire act: The poised genome and cellular memory | FEBSJ, 282(9):1675-91. doi: 10.1111/febs.13165 | 2014 |
| Deep sequencing reveals unique small RNA repertoire that is regulated during head regeneration in Hydra magnipapillata | Nucleic Acids Research, 41(1):599-616. doi: 10.1093/nar/gks1020 | 2013 |
| Applications of Microfabrication and Microfluidic Techniques in Mesenchymal Stem Cell Research | Microfluidic Technologies for Human Health, 69-95. doi: 10.1142/9789814405522_0004 | 2013 |
| Vertebrate GAGA factor associated insulator elements demarcate homeotic genes in the HOX clusters | Epigenetics & Chromatin, 6(1):8. doi: 10.1186/1756-8935-6-8 | 2013 |
| A novel in vitro model for studying quiescence and activation of primary isolated human myoblasts | PLOS One, 8(5):e64067. doi: 10.1371/journal.pone.0064067 | 2013 |
| Distinct transcriptional networks in quiescent myoblasts: a role for Wnt signaling in reversible vs. irreversible arrest | PLOS One, 8(6):e65097. doi: 10.1371/journal.pone.0065097 | 2013 |
| Regulation of cellular chromatin state: insights from quiescence and differentiation | Organogenesis, 6(1):37-47 | 2010 |
| The paternal hidden agenda: Epigenetic inheritance through sperm chromatin | Epigenetics, 5(5):386-91 | 2010 |
| MLL5, a trithorax homolog, indirectly regulates H3K4 methylation, represses Cyclin A2, expression, and promotes myogenic differentiation | PNAS, 106(12):4719-24. doi: 10.1073/pnas.0807136106 | 2009 |
| The small chromatin protein p8/Nupr1 co-ordinates anti-proliferative and pro-myogenic proteins targeting the Myogenin promoter | Journal of Cell Science, 122: 3481-3491; doi: 10.1242/jcs.048678 | 2009 |
| Consensus Guidance for Banking and Supply of Human Embryonic Stem Cell Lines for Research Purposes - International Cell Banking Initiative | Stem Cell Reviews and Reports, 5(4):301-14. doi: 10.1007/s12015-009-9085-x | 2009 |
| A gene trap strategy identifies quiescence induced genes in synchronized myoblasts | Journal of Biosciences, 33(1):27-44 | 2008 |
| The RhoA effector mDiaphanous regulates MyoD expression and cell cycle progression via SRF-dependent and SRF-independent pathways | Journal of Cell Science, 120 (Pt 17):3086-98. doi: 10.1242/jcs.006619 | 2007 |
| Modulation of AP and DV signalling pathways by the homeotic gene Ultrabithorax during haltere development in Drosophila | Developmental Biology, 291(2):356-67, doi: 10.1016/j.ydbio.2005.12.022 | 2006 |
| Stem Cells in Postnatal Myogenesis: Molecular Mechanisms of Satellite Cell Quiescence, Activation and Replenishment | Trends in Cell Biology, 15(12):666-73, doi: 10.1016/j.tcb.2005.10.007 | 2005 |
| Modulation of acto-myosin contractility in skeletal muscle myoblasts uncouples growth arrest from differentiation | Journal of Cell Science, 117(Pt 17):3735-48. doi: 10.1242/jcs.01197 | 2004 |
| Skeletal Muscle Progenitor Cells in Development and Regeneration | Proc. Indian Natn. Sci. Acad, B69 (5) 719-740 | 2003 |
| Tristetraprolin and LPS-inducible CXC chemokine are transiently induced in presumptive satellite cells in response to skeletal muscle injury | Journal of Cell Science, 115 (13) 2701-2712 | 2002 |
| Distinct changes in intranuclear lamin A/C organization during myoblast differentiation. | Journal of Cell Science, 114: 4001-4011 | 2001 |
2544
jdhawan@ccmb.res.in
priti@ccmb.res.in
List of shortlisted candidates for the final round of Interview for PhD Jan 2025 session