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Krishnan H Harshan

Principal Scientist

Research Interests

I currently use two different viral systems for studies: HCV is a positively sensed RNA virus which is transmitted across humans through blood. HCV infection could lead to chronic infection in more than 70% of the infected persons, which ultimately results in the development of liver cirrhosis (LC) and hepatocellular carcinoma (HCC). There are no vaccines available against HCV infection and the current treatment regime uses pegylated interferon a with ribovirin. The studies in the lab focus on various signaling pathways induced and manipulated by HCV in hepatocytes. KSHV, a g-herpesvirus has been implicated in the development of Kaposi’ sarcoma which is an angiomatous neoplasm seen in immune suppressed persons such as AIDS patients. Unlike other herpesviruses, KSHV does not induce a true lytic cycle in vitro, however induces an aberrant lytic cycle, and establishes latency. The mechanism by which true lytic cycle is inhibited early on in the infection is being analyzed in his laboratory.

Selected Publications

Krishnan, H.H., et al., 2004. Concurrent expression of latent and a limited number of lytic genes with immune modulation and anti-apoptotic functions by KSHV/HHV-8 early during infection of primary endothelial and fibroblast cells and subsequent decline of lytic gene expression. J Virology: 78:3601-20.

George, A., et al., 2012. Hepatitis C virus NS5A binds to the mRNA Cap binding eIF4F complex and upregulates host translation initiation machinery through 4EBP1 inactivation. J. Biol. Chemistry. 10; 287(7): 5042-58.

Panda, S., et al., 2014. Hepatitis C Virus Protein NS5A Coordinates a Unique Phosphorylation Dependent eIF4E Assembly on 40S Ribosomes That Regulates IRES Activity. Biochemical Journal, Sep 1;462(2):291-302.

Vedagiri, D., et al., 2016. An Atypical System for Studying Epithelial-Mesenchymal Transition in Hepatocellular Carcinoma. Scientific Reports 6:26282

Parveen, S., et al., 2017. Unconventional MAPK-GSK-3? Pathway Behind Atypical Epithelial-Mesenchymal Transition In Hepatocellular Carcinoma. Scientific Reports 7, 8842 (2017)

 

Education & Experiance

P.G: Biotechnology ; Cochin University of Science and Technology ; 1998
Ph.D: Anaerobic regulation in V.cholerae ; Jadavpur/Indian Institute of Chemical Biology ; 2003
Post.Doc:

Virology ; University of Kansas Medical Center ; 2002-2007

Publications

TitleJournalYear
mTORC1 Restricts Hepatitis C Virus RNA Replication Through ULK1-mediated Suppression of miR-122 and Facilitates Post-replication Events. Journal of General Virology (2019). DOI 10.1099/jgv.0.001356 2019
An Atypical System for Studying Epithelial-Mesenchymal Transition in Hepatocellular Carcinoma. Sci Rep. 2016 May 20;6:26282. doi: 10.1038/srep26282. 2016
Hepatitis C Virus Protein NS5A Coordinates a Unique Phosphorylation Dependent eIF4E Assembly on 40S Ribosomes That Regulates IRES Activity. Biochemical Journal 2014 Jun 4. PMID:24894874; doi:10.1042/BJ20131530). 2014
Hepatitis C virus NS5A binds to the mRNA Cap binding eIF4F complex and upregulates host translation initiation machinery through 4EBP1 inactivation. Journal of Biological Chemistry. Journal of Biological Chemistry. 287, NO. 7, 5042 2011
Focal Adhesion Kinase (FAK) is critical for Kaposi's Sarcoma Associated Herpesvirus (KSHV-HHV-8) entry into the target cells. J. Virology. 80. 1167-1180. (Selected in the J.Virology spotlight in the same issue) 2006
Envelope glycoprotein gB of Kaposi's sarcoma Associated Herpesvirus (KSHV/HHV-8) is essential for egress from infected cells J. Virology. 79. 10952-10967. 2005
ERK1/2 and MEK1/2 induced by Kaposi's Sarcoma Associated Herpesvirus (KSHV/HHV-8) early during infection of target cells are essential for the expression of viral genes and for the establishment of infection. J. Virology. 79. 10308-10329. 2005
Kaposi's sarcoma associated herpesvirus (KSHV/HHV-8) modulates the Microtubule Dynamics via RhoAGTP- Diaphenous-2 Signaling and Utilizes the Dynein Motors to deliver its DNA to the Nucleus. J. Virology. 79. 1191-1206. 2005
Effect of anaerobiosis on expression of virulence factors in Vibrio cholerae. Infection and Immunity. 72. 3961-3967. 2004
Kaposi's Sarcoma Associated Herpesvirus (KSHV/HHV-8) envelope glycoprotein gB induces the integrin dependent focal adhesion kinase-Src-PI-3K-RhoGTPase signal pathways and cytoskeletal rearrangements. J.Virology. 78: 4207-4223. 2004
Concurrent expression of latent and a limited number of lytic genes with immune modulation and anti-apoptotic functions by KSHV/HHV-8 early during infection of primary endothelial and fibroblast cells and subsequent decline of lytic gene expression. J Virology: 78:3601-20. 2004
Host gene induction and transcriptional reprogramming in KSHV/HHV-8 infected endothelial, fibroblast and B cells: Insights into modulation events early during infection. Cancer Research; 64:72-84. 2004
Kaposi's sarcoma-associated herpesvirus induces Phosphatidylinositol 3-Kinase-PKC-?-MEK-ERK signaling pathway in the target cells early during infection: implications in infectivity J.Virology. 77:1524-1539. 2003

Krishnan H Harshan

Principal Scientist

2925

hkrishnan@ccmb.res.in

Mohan Singh M

Technical Officer

2930

mohan_singh@ccmb.res.in

Amit Kumar

Technical Officer

2950

amitkumar@ccmb.res.in

Nivedita Gaur

Research Associate-I (DBT)

2930

nivedita@ccmb.res.in

Dhiviya Vedagiri

Senior Research Fellow

2930

dhiviya@ccmb.res.in

Sana Parveen

Senior Research Fellow

2930

parveen@ccmb.res.in

Divya Gupta

Senior Research Fellow

2930

divyagupta@ccmb.res.in

Haripriya Parthasarathy

Senior Research Fellow

2930

haripriya@ccmb.res.in

Vishal Sah

Junior Research Fellow

2930

vishal.sah@ccmb.res.in

Dixitkumar Tandel

Junior Research Fellow

2930

dixitkumar@ccmb.res.in