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Lekha Dinesh Kumar

Senior Principal Scientist

Research Interests

Our laboratory is interested in studying the changes induced, with an emphasis on wnt signal transduction pathway, during the initiation and progression of colon and breast cancer. wnt deregulators are identified by molecular and in vitro cell culture methods and their role in both induction and progression of early lesions are tested by generating transgenic murine models. These models could also serve as an in vivo drug validation and testing models of colorectal neoplasia. Another project of interest in our laboratory is to look at the role of microRNAs (oncomiRs) in the development of colon and breast cancer. OncomiRs, a special class of non-coding microRNAs are found to be associated with a large number of cancers. We have developed a microRNA database for breast and colon cancer and experimentally tested these on different microarray platforms. Impaired microRNA signatures are noticed in different stages and grades of these tumours. We are further characterizing the microRNA targets for developing into functional biomarkers.

Selected Publications

Rekha Nair#, Vinod kumar Verma#, Syed Beevi+, Abdul Rawoof+, Liza Alexander+, Ramanjaneya Prasad, Kusuma Kumari, Prashant Kumar, Lekha Dinesh Kumar*. (2020).  MicroRNA signatures in blood or bone marrow distinguish subtypes of pediatric Acute Lymphoblastic Leukemia. Translational Oncology  13 (2020) 100800  http://dx.doi.org/10.1016/j.tranon.2020.100800

Abdul Rawoof, Guruprasadh Swaminathan, Shrish Tiwari, Rekha A Nair, and *Lekha Dinesh Kumar (2020). LeukmiR: A database for miRNAs and their targets in Acute Lymphoblastic Leukemia. (2020). DATABASE (Oxford press). Vol: 2020 1-11 :baz151. doi: 10.1093/database/baz151. PMID: 32128558 

Souneek Chakraborty,  Aviral Kumar, Anmol Kumar , Debasis Nayak, Aparna Golani, JedyJose ,*Lekha Dinesh Kumar  and *Anindya Goswami (2019).
Vimentin activation in early apoptotic cancer cells errands survival pathways during DNA damage inducer CPT treatment in colon carcinoma model. Cell Death and Disease .10:467
  https://doi.org/10.1038/s41419-019-1690-2 

Dinesh Kumar L. (eds) In: RNA Interference and Cancer Therapy. Methods in Molecular Biology, Springer Protocols (2019). vol 1974. Humana, New York, NY. doi:https://doi.org/10.1007/978-1-4939-9220-1_193.

Tangudu NK, Verma VK, Clemons TD, Beevi SS, Hay T, Mahidhara G, Raja M, Nair RA, Alexander LE, Patel AB, Jose J, Smith NM, Zdyrko B, Bourdoncle A, Luzinov I, Iyer KS, Clarke AR, *Dinesh Kumar L. (2015). RNA Interference Using c-Myc-Conjugated Nanoparticles Suppresses Breast and Colorectal Cancer Models. Molecular Cancer Therapeutics. 14 (5):1259-69. doi: 10.1158/1535-7163.MCT-14-0970. Epub 2015 Feb 18. PMID: 25695957. IF 4.8

Education & Experiance

P.G: 1.Zoology,Kerala University (1985-87) ; 2. Molecular Biology & Biotechnology, IARI, New Delhi (1987-89) ;
Ph.D: Molecular Biology & Biotechnology ; IARI, New Delhi ; 1995
Isolation,cloning and characterization of genomic clones encoding the legumin storage proteins in chick pea (Cicer arietinum L.).
Post.Doc:

Vicky Dickinson Memorial Fellowship ; School of Biosciences,Cardiff University,United Kingdom (2005) ;
Cancer Biology,Transgenic knock-in and knock-out murine cancer models

Experience:

Visiting Scientist, School of Biosciences,Cardiff University,United Kingdom as UICC awardee (International Union Against Cancer),(2008)
Visiting Scientist,University of Western Australia,Perth, Australia (2009)

Patents

InventionCountries of filing the patentApplication numberPublication numberStatusGrant Number
Biomarkers useful for detection of grades of human breast cancer India 1142/DEL/2011 Granted & Active 313602
Biomarkers useful for detection of types, grades and stages of human breast cancer UK 1408696.1, 1603127.0, 1603128.8, 1608839.5, 1608844.5 WO2013/057567 Granted & Active GB 2509682B, GB 2535914 B, GB 2536374 B, GB 2533872 B, GB 2533873 B
Biomarkers useful for detection of types, grades and stages of human breast cancer. United States of America 16/219,572 W O 2013/057567 Al, Dated: 25 April 2013 Filed/final stage Under process
Biomarkers useful for detection of types, grades and stages of human breast cancer. Canada PD007546CA-NP, 3077750 W O 2013/057567 Al , Dated: 25 April 2013 Granted & Active CA2852384A1
Biomarkers useful for detection of types, grades and stages of human breast cancer. Australia AU 2012324531 B2 W O 2013/057567 Al , Dated: 25 April 2013 Filed Under process
Biomarker Discovery in Paediatric Acute Lymphoblastic Leukemia India 2963/DEL/2013 10/04/2015 Filed & Published Under process

Publications

TitleJournalYear
MicroRNA signatures in blood or bone marrow distinguish subtypes of pediatric Acute Lymphoblastic Leukemia. TranslationalOncology 13 (2020) 100800 http://dx.doi.org/10.1016/j.tranon.2020.100800 2020
EMT in breast cancer metastasis: an interplay of microRNAs, signaling pathways and circulating tumor cells. Frontiers in Biosciences : Landmark edition : 25 (5) : 979-1010 doi: http://www.bioscience.org/2020/v25/af/4844/fulltext.htm 2020
LeukmiR: A database for miRNAs and their targets in Acute Lymphoblastic Leukemia. DATABASE (Oxford press). Vol: 2020 1-11 :baz151. doi: 10.1093/database/baz151. PMID: 32128558 2020
Biodrug Suppresses Breast and Colorectal Cancer in Murine Models. In: RNA Interference and Cancer Therapy. Methods in Molecular Biology, Springer Protocols (2019) vol 1974. Humana, New York, NY. Dinesh Kumar L. (eds) doi:https://doi.org/10.1007/978-1-4939-9220-1_19 2019
RNA Interference and Cancer Therapy. Methods in Molecular Biology, Springer Protocols (2019) vol 1974. Humana, New York, NY. doi:https://doi.org/10.1007/978-1-4939-9220-1_19 2019
Vimentin activation in early apoptotic cancer cells errands survival pathways during DNA damage inducer CPT treatment in colon carcinoma model. Cell Death and Disease .10:467 https://doi.org/10.1038/s41419-019-1690-2 2019
Dual role of Par-4 in abrogation of EMT and switching on Mesenchymal to Epithelial Transition (MET) in metastatic pancreatic cancer cells. Mol Carcinog. 2018 Apr 19. doi: 10.1002/mc.22828. [Epub ahead of print] PMID:29672923 2018
Inhibition of Twist1-mediated invasion by Chk2 promotes premature senescence in p53-defective cancer cells Cell Death and Differentiation (2017),1-13 (Advance online publication, 12 May 2017; doi:10.1038/cdd.2017.70) 2017
AKT is indispensable for coordinating Par-4/JNK cross talk in p21 downmodulation during ER stress Oncogenesis(2017)6,e341; doi:10.1038/oncsis.2017.41 2017
Dual modulation of Ras-Mnk and PI3K-AKT-mTOR pathways: A Novel c-FLIP inhibitory mechanism of 3-AWA mediated translational attenuation through dephosphorylation of eIF4E. Scientific Reports 2016
PAWR-mediated suppression of BCL2 promotes switching of 3-azido withaferin A(3-AWA)-induced autophagy to apoptosis in prostate cancer cells Autophagy 11:2, 314-331; February 2015 2015
RNA Interference using c-Myc-Conjugated Nanoparticles Suppresses Breast and Colorectal Cancer Models. Mol Cancer Ther. 2015 May;14(5):1259-69. doi: 10.1158/1535-7163. 2015
Par-4 dependent modulation of cellular ?-catenin by medicinal plant natural product derivative 3-azido Withaferin A. Molecular Carcinogenesis. 2015 May 12. doi: 10.1002/mc.22328 2015
OncomiRdbB: a comprehensive database of microRNAs and their targets in breast cancer BMC Bioinformatics 2014, 15:15 (15 January 2014) Database-link, 2014
Gene manipulation through the use of small interfering RNA (siRNA): from in vitro to in vivo applications. Advance Drug Delivery Reviews, 59 : 87-100. 2007

Lekha Dinesh Kumar

Senior Principal Scientist

2933

lekha@ccmb.res.in