CSIR - Centre for Cellular & Molecular Biology
Council of Scientific and Industrial Research
Ministry of Science & Technology, Govt. of India
Senior Principal Scientist
Email: psijwali@ccmb.res.in
Phone: +91-040-27192944
Malaria parasite has a wonderfully complex development that involves 15 different forms, disguising the host defence arsenal, to complete its life cycle. Many of these forms reside in different cellular environments and contain stage-specific proteins and functionally specialized organelles, which are produced and disposed off as and when required. Additionally, the parasite not only breaks down host cellular contents to obtain nutrients, it also enslaves many host proteins for its benefit. My lab is investigating the ubiquitin proteasome and lysosomal pathways of malaria parasites, which likely regulate parasite development and disease pathogenesis through degradative and non-degradative functions. We combine gene knock-out/knock-in/knock-down approaches with biochemical and cell biology methods in our studies on both P. falciparum and in vivo models of malaria. We also have a high throughput screening assay for anti-malarial drug discovery.
Selected Publications
Divya N. Nair, Rajesh Prasad, Neha Singhal, Manish Bhattacharjee, Renu Sudhakar, Pushpa Singh, Subramonian Thanumalayan, Uday Kiran, Yogendra Sharma and Puran Singh Sijwali. (2018) A conserved human DJ1-subfamily motif (DJSM) is critical for anti-oxidative and deglycase activities of Plasmodium falciparum DJ1. Molecular & Biochemical Parasitology. 222:70-80. doi:10.1016/j.molbiopara.2018.05.003
Kolla VK, Prasad R, Sayyad Z, Atul, Shah AY, Allanki AD, Navale R, Singhal N, Tanneru N, Sudhakar R, Venkatesan V, Deshmukh MV and Sijwali PS. (2015) Independent amino acid residues in the S2 pocket of falcipain-3 determine its specificity for P2 residues in substrates. Mol Biochem Parasitol. 202(2):11-22. doi: 0.1016/j.molbiopara.2015.09.005.
Education & Experience
P.G: | Molecular Biology and Biotechnology ; Govind Ballabh Pant University of Agriculture & Technology, Pantnagar, India ; 1994 |
Ph.D: | Cloning and characterization of the thrombospondin-related adhesive protein (TRAP) from the simian and the human malaria parasites ; International Centre for Genetic Engineering and Biotechnology-Jawaharlal Nehru University, New Delhi, India ; 1999 |
Post.Doc: | Plasmodium proteases and hemoglobin degradation in erythrocytes ; UNIVERSITY OF CALIFORNIA SAN FRANCISCO, CA, USA ; 1999-2004 |
Experience: | Assistant Researcher at UNIVERSITY OF CALIFORNIA SAN FRANCISCO, CA, USA from 2004-2008 |
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Title | Journal | Year |
---|---|---|
Bazedoxifene, a Postmenopausal Drug, Acts as an Antimalarial and Inhibits Hemozoin Formation | Microbiol Spectr | 2022 |
Dissecting Plasmodium yoelii Pathobiology: Proteomic Approaches for Decoding Novel Translational and Post-Translational Modifications | ACS Omega | 2022 |
Plasmodium falciparum Atg18 localizes to the food vacuole via interaction with the multi-drug resistance protein 1 and phosphatidylinositol 3-phosphate | Biochemical Journal | 2021 |
Characterization of Plasmodium falciparum NEDD8 and identification of cullins as its substrates | Scientific Reports | 2020 |
Identification of antimalarial leads with dual falcipain-2 and falcipain-3 inhibitory activity | Bioorg Med Chem Lett | 2020 |
Synthesis and efficacy of pyrvinium-inspired analogs against tuberculosis and malaria pathogens | Bioorg Med Chem Lett | 2020 |
Lyse-Reseal Erythrocytes for Transfection of Plasmodium falciparum | Scientific Reports | 2019 |
Plasmodium falciparum GCN5 acetyltransferase follows a novel proteolytic processing pathway essential for its function | Journal of Cell Science | 2019 |
Interaction with complement proteins and dendritic cells implicates LCCL domain-containing proteins (CCps) of malaria parasites in immunomodulation | Biochemical Journal | 2018 |
Biotransformation of Artemisinin to 14-hydroxydeoxyartemisinin: C-14 hydroxylation by Aspergillus flavus. | Journal of Agricultural and Food Chemistry | 2018 |
A conserved human DJ1-subfamily motif (DJSM) is critical for anti-oxidative and deglycase activities of Plasmodium falciparum DJ1. | Molecular & Biochemical Parasitology. 222:70-80. doi:10.1016/j.molbiopara.2018.05.003 | 2018 |
Biotransformation of Artemisinin to 14-hydroxydeoxyartemisinin: C-14 hydroxylation by Aspergillus flavus. | Journal of Agricultural and Food Chemistry. DOI: 10.1021/acs.jafc.8b03573 | 2018 |
Synthesis and evaluation of anti-plasmodial and cytotoxic activities of epoxyazadiradione derivatives. | Eur J Med Chem | 2017 |
Synthesis and evaluation of anti-plasmodial and cytotoxic activities of epoxyazadiradione derivatives. | Eur J Med Chem. 134:242-257. doi: 10.1016/j.ejmech.2017.04.016. | 2017 |
A Novel and conserved Plasmodium sporozoite membrane protein SPELD is required for maturation of exo-erythrocytic forms. | Sci Rep.7: 40407. doi: 10.1038/srep40407. | 2017 |
Synthesis and evaluation of naphthyl bearing 1,2,3-triazole analogs as antiplasmodial agents, cytotoxicity and docking studies. | Bioorg Med Chem. 2017;25(1):221-232. doi: 10.1016/j.bmc.2016.10.029. | 2016 |
Independent amino acid residues in the S2 pocket of falcipain-3 determine its specificity for P2 residues in substrates. | Mol Biochem Parasitol. 202(2):11-22. doi: 0.1016/j.molbiopara.2015.09.005. | 2015 |
Emergence of pyrido quinoxalines as new family of antimalarial agents. | Eur J Med Chem. 77C:280-287. | 2014 |
Genetic ablation of plasmoDJ1, a multi-activity enzyme, attenuates parasite virulence and reduces oocyst production. | Biochem. J. 461 (189-203). | 2014 |
Characterization of the autophagy marker protein Atg8 reveals atypical features of autophagy in Plasmodium falciparum. | PLoS One 9(11): e113220. doi:10.1371/journal.pone.0113220. | 2014 |
Synthesis and insight into the structure-activity relationships of chalcones as antimalarial agents. | J Med Chem. 2013 Jan 10;56(1):31-45. | 2013 |
Blocking Plasmodium falciparum development via dual inhibition of hemoglobin degradation and the ubiquitin proteasome system by MG132. | PLoS One. 8(9):e73530. | 2013 |
The Ionic and Hydrophobic Interactions Are Required for the Auto Activation of Cysteine Proteases of Plasmodium falciparum. | PLoS One. 7(10):e47227. | 2012 |
Expression, Characterization, and Cellular Localization of Knowpains, Papain-Like Cysteine Proteases of the Plasmodium knowlesi Malaria Parasite. | PLoS One. 2012;7(12):e51619. | 2012 |
Functional evaluation of Plasmodium export signals in Plasmodium berghei suggests multiple modes of protein export. | PLoS One 19;5(4): e10227. | 2010 |
Antimalarial effects of human immunodeficiency virus type 1 protease inhibitors differ from those of the aspartic protease inhibitor pepstatin. | Antimicrob Agents Chemother 50(6): 2207-2209. | 2007 |
Falcipain cysteine proteases require bipartite motifs for trafficking to the Plasmodium falciparum food vacuole. | Journal of Biological Chemistry 282(34):24961-9. | 2007 |
A chimeric cysteine protease of Plasmodium berghei engineered to resemble the Plasmodium falciparum protease falcipain-2. | Protein Engineering, Design and Selection 20(4):171-177. | 2007 |
Gene disruptions demonstrate independent roles for the four falcipain cysteine proteases of Plasmodium falciparum. | Mol Biochem Parasitol 150(1): 96:106. | 2006 |
Structural basis for unique mechanisms of folding and hemoglobin binding by a malarial protease. | Proceedings of the National Academy of Sciences USA 103(31):11503-8. | 2006 |
Plasmodium falciparum: Biochemical characterization of the cysteine protease falcipain-2'. | Exp Parasitol 112(3):187-192. | 2005 |
The Plasmodium falciparum cysteine protease falcipain-2 captures its substrate, hemoglobin, via a unique motif. | Proceedings of the National Academy of Sciences USA102(26): 9138-9143. | 2005 |
Plasmodium falciparum cysteine protease falcipain-1 is not essential in erythrocytic stage malaria parasites. | Proceedings of the National Academy of Sciences USA 101(23): 8721-8726. *Equal Contribution. | 2004 |
Identification and biochemical characterization of vivapains, cysteine proteases of the malaria parasite Plasmodium vivax. | Biochemical Journal 378(2): 529-538. | 2004 |
Gene disruption confirms a critical role for the cysteine protease falcipain-2 in hemoglobin hydrolysis by Plasmodium falciparum. | Proceedings of the National Academy of Sciences USA 101(13): 4384-4389. | 2004 |
Independent intramolecular mediators of folding, activity, and inhibition for the Plasmodium falciparum cysteine protease falcipain-2. | Journal of Biological Chemistry 279(5): 3484-3491. | 2004 |
Critical role of amino acid 23 in mediating activity and specificity of vinckepain-2, a papain-family cysteine protease of rodent malaria parasites. | Biochemical Journal 368(1): 273-281. | 2002 |
Folding of the Plasmodium falciparum cysteine protease falcipain-2 is mediated by a chaperone-like peptide and not the prodomain. | Journal of Biological Chemistry 277(17): 14910-14915. | 2002 |
Expression and characterization of the Plasmodium falciparum haemoglobinase falcipain-3. | Biochemical Journal 360(2): 481-489. | 2001 |
Recombinant falcipain-2 cleaves erythrocyte membrane ankyrin and protein 4.1. | Molecular Biochemical Parasitology 116(1): 95-99. | 2001 |
Systematic optimization of expression and refolding of the Plasmodium falciparum cysteine protease falcipain-2. | Protein Expression and Purification 22(1): 128-134. | 2001 |
Systematic optimization of expression and refolding of the Plasmodium falciparum cysteine protease falcipain-2. | Protein Expression and Purification 22(1): 128-134. | 2001 |
Characterization of native and recombinant falcipain-2, a principal trophozoite cysteine protease and essential hemoglobinase of Plasmodium falciparum. | Journal of Biological Chemistry 275(37): 29000-29010. *Equal Contribution. | 2000 |
Cloning and sequence analysis of the thrombospondin-related adhesive protein (TRAP) gene of Plasmodium cynomolgi bastianelli. | Molecular Biochemical Parasitology 90(1): 371-376. | 1997 |
Detection and quantitation of Helicoverpa armigera nuclear polyhedrosis virus by ELISA technique. | Indian Journal of Microbiology 36: 229-230. | 1996 |
2944
psijwali@ccmb.res.in
2928
deepak.kumar@ccmb.res.in
2928
zeba@ccmb.res.in
2928
g.srinivasreddy@ccmb.res.in
2928
gordesomesh@ccmb.res.in
2928
manash@ccmb.res.in
pritamdas@ccmb.res.in
2940
kanikasaxena@ccmb.res.in