First slide

Raghunand R Tirumalai

Principal Scientist

Research Interests

Mycobacterium tuberculosis (M.tb) the causative organism of human tuberculosis is responsible for almost 2 million deaths annually. Even though the organism was first identified as being the cause of TB over a century ago, our understanding of the molecular basis of its pathogenicity remains nebulous. Treatment of cases requires multi-drug therapy for a minimum of 4-6 months and control of M.tb infection is complicated by the complex long-term nature of the host-pathogen interactions. Most often, the subsequent development of specific immunity results in ‘latent’ infection, and reactivation tuberculosis can therefore occur years after initial exposure. Our research group is focussed towards understanding the molecular basis of Latency, the identification of mycobacterial virulence factors and the discovery of novel targets for the development of anti-TB therapeutics.

Selected Publications

Yeruva, V. C., Kulkarni, A. M., Khandelwal, R., Sharma, Y. and Raghunand, T. R. (2016). The PE_PGRS proteins of Mycobacterium tuberculosis are Ca2+ binding mediators of host-pathogen interaction. Manuscript accepted for publication, Biochemistry(ACS)

Sharma S.G.S., Kishore, V. and Raghunand T. R. (2016) Identification and functional annotation of mycobacterial septum formation genes using cell division mutants of Escherichia coli. Res Microbiol, 167(2):142-8

Viswanathan, G., Yadav, S. and Raghunand, T. R (2016) Identification of Novel Loci Associated with Mycobacterial Isoniazid Resistance. Tuberculosis 96:21-26

Viswanathan, G., Joshi, S. V., Sridhar, A., Dutta, S. and Raghunand, T. R (2015) Identifying novel mycobacterial stress associated genes using a random mutagenesis screen in Mycobacterium smegmatis. Gene 574(1):20-27

Tiwari, B., Maheswari R. U. and Raghunand, T. R. (2015) The Mycobacterium tuberculosis protein pair PE9 (Rv1088) - PE10 (Rv1089) forms heterodimers and induces macrophage apoptosis through TLR4. Cellular Microbiol 17(11): 1653-1669


Education & Experiance

P.G: Biotechnology ; Indian Institute of Technology, Bombay, India ; 1994
Ph.D: Molecular Analysis of beta-glucoside utilisation in Klebsiella aerogenes ; Indian Institute of Science, Bangalore, India ; 2001
Post.Doc:

Mycobacterial Pathogenesis ; Johns Hopkins School of Medicine, Baltimore, USA ; 2001-2007

Publications

TitleJournalYear
Macrophage sphingolipids are essential for the entry of mycobacteria Chem Phys Lipids 213:25-31 2018
Insights into the function of FhaA, a cell-division associated protein in mycobacteria. FEMS Microbiology Letters 364 (2):fnw294 2017
Identification of mycobacterial genes involved in ?-lactam sensitivity - Implications in treatment of tuberculosis with ?-lactam containing regimens Antimicrobial Agents and Chemotherapy 61(7) e00425-17. https://doi .org/10.1128/AAC.00425-17 2017
Identification of Novel Loci Associated with Mycobacterial Isoniazid Resistance Tuberculosis 96:21-26 2016
The PE_PGRS proteins of Mycobacterium tuberculosis are Ca2+ binding mediators of host-pathogen interaction Biochemistry(ACS)55(33):4675-87 2016
Identification and functional annotation of mycobacterial septum formation genes using cell division mutants of Escherichia coli Res Microbiol, 167(2):142-8 2016
The Mycobacterium tuberculosis desaturase DesA1 (Rv0824c) is a Ca2+ binding protein Biochem Biophys Res Comm 480(1):29-35 2016
The PE_PGRS proteins of Mycobacterium tuberculosis are Ca2+ binding mediators of host-pathogen interaction Manuscript accepted for publication, Biochemistry (ACS) 2016
The Mycobacterium tuberculosis protein pair PE9 (Rv1088) - PE10 (Rv1089) forms heterodimers and induces macrophage apoptosis through TLR4 Cellular Microbiol 17(11): 1653-1669 2015
Dissecting the Membrane Cholesterol Requirement for Mycobacterial Entry into Host Cells Chem Phys Lipids 189:19-27 2015
Identifying novel mycobacterial stress associated genes using a random mutagenesis screen in Mycobacterium smegmatis Gene 574(1):20-27 2015
An immunomodulatory role for the Mycobacterium tuberculosis RD1 locus proteins PE35 (Rv3872) and PPE68 (Rv3873) Febs J 281:1556-70 2014
The Mycobacterium tuberculosis PE Proteins Rv0285 and Rv1386 Modulate Innate Immunity and Mediate Bacillary Survival in Macrophages PLoS ONE 7(12): e51686. doi:10.1371/journal.pone.0051686 2012
Characterization of clioquinol and analogues as novel inhibitors of methionine aminopeptidases from Mycobacterium tuberculosis Tuberculosis (Edinb) 91S1:S61-S65. Epub 2011 Nov 23 2011
Methionine Aminopeptidases from Mycobacterium tuberculosis as Novel Antimycobacterial Targets Chemistry and Biology. 17(1):86-97 2010
A Reproducible Bronchoscopic Model of Cavitary Tuberculosis Using Tuberculin-Sensitized Infected Outbred Rabbits Infect.& Immunity.77(2):598-603 2008
Mapping essential domains of Mycobacterium smegmatis WhmD: Insights into WhiB structure and function J. Bacteriol.188 (19): 6966-6976 2006
Mycobacterium smegmatis whmD and its homologue Mycobacterium tuberculosis whiB2 are functionally equivalent. Microbiology 152(9): 2735-2747 2006
Towards establishing a method to screen for inhibitors of essential genes in mycobacteria: Evaluation of the Acetamidase promoter Int. J. Antimicrob. Agents 28(1): 37-42 2006
Regulation of the expression of whiB1 in Mycobacterium tuberculosis: Role of cAMP receptor protein Microbiology 152(9): 2749-2756 2006
Differential gene expression in response to antibiotic exposure, growth phase, and stress conditions among the seven Mycobacterium tuberculosis whiB-like genes Antimicrob. Agents Chemother. 50(8):2836-2841 2006
Deletion of a Mycobacterium tuberculosis proteasomal ATPase homologue produces a slow growing strain which persists in host tissues J. Infect. Dis.194:1233-1240 2006
Mutational analysis of ?-glucoside utilisation in Klebsiella aerogenes: evidence for the presence of multiple genetic systems J. Genetics. 83(3): 285-289 2004
The beta-glucoside genes of Klebsiella aerogenes: conservation and divergence in relation to the cryptic bgl genes of Escherichia coli. FEMS Microbiol. Lett. 223(2):267-74 2003
Transgene Silencing: New insights into an old puzzle. J Biosciences 23(5): 539-540 1998
Characterisation of 3’ end formation in the yeast HIS3 mRNA Gene 190:69-76 1997
Molecular clues to the control of circadian rhythms. Resonance 1(4): 76-81 1996

Raghunand R Tirumalai

Principal Scientist

2924

raghu@ccmb.res.in

Ravi Prasad Mukku

Senior Research Fellow

2930

raviprasad.mukku@ccmb.res.in