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Research Interests

Stem Cell Biology and Tissue Engineering Basic biology of Adult multipotent stem cells from different sources and iPS eneration, Differentiation of the multipotent cells into various lineages using cues from nanomaterials, modified surfaces, growth factors or natural compounds.

Selected Publications

 

Jennifer Xavier, Shashi Singh, David A Dean, N. Madhusudhana Rao, Vijaya Gopal (2008) Designed multi-domain protein as a carrier of nucleic acids into cells. Journal of Controlled Release 133 (2009) 154–160

 

Susinjan Bhattacharya, Naveena Lavanya Latha. J, R. Kumresan and Shashi Singh (2007)“Cloning And Expression Of Human Islet Amyloid Polypeptide In Cultured Cells. Biochemical and Biophysical Research Communications 356 (2007) 622–628

 

Vemuganti GK, Kashyap S, Sangwan VS, Singh S. (2004) Ex-vivo potential of cadaveric and fresh limbal tissues to regenerate cultured epithelium. Indian J Ophthalmol 52:113-20.

 

Virender S Sangwan, Geeta Kashyap Vemuganti, Shashi Singh, D.Balasubramanian (200

 

Education & Experiance

P.G: Zoology ; University of Delhi ; 1982
Ph.D: Ultrastructural studies of encystment and excystment in Stylonychia mytilis and Pseudourostyla lemni. ; UNiversity of Delhi ; 1988
Experience:

Junior research Officer NII 1987-1988 Lecturer University of Roorkee Sept 1988- NOv 1988 Scientist in CCMB Dec 1988

Publications

TitleJournalYear
Calcium-phosphate nanobioceramics. Letters in Applied NanoBioScience vol5 389-396 2016
An interlaboratory comparison of nanosilver characterisation and hazard identification: Harmonising techniques for high quality data Environment International 87 20-32 2016
A harmonised protocol for testing the toxicity of nanomaterials with brine shrimp Artemia sp. Environmental Pollution, 213, 173-183 2016
Multilaboratory evaluation of 15 bioassays for (eco)toxicity screening and hazard ranking of engineered nanomaterials: FP7 project NANOVALID. Nanotoxicology, DOI: 10.1080/17435390.2016.1196251[6.411] 2016
Assessment of injectable and cohesive nanohydroxyapatite composites for biological functions. Progress in Biomaterials 4:31-38 2015
Evaluation of nano-biphasic calcium phosphate ceramics for bone tissue engineering applications: In vitro and preliminary in vivo studies. [2.246] {citations 4} Journal of Biomaterials Applications; 27(5):565-75. doi: 10.1177/0885328211415132 2013
TiO2 nanoparticles induce oxidative DNA damage and apoptosis in human liver cells Nanotoxicology.7:48-60 (doi: 10.3109/17435390.2011.629747) 2013
Synthetic Adhesive Oligopeptides with Rigid Polyhydroxylated Amino Acids. Biopolymers 99:273-281 2013
Poly (L-Lysine)-pyranine-3 coacervate mediated nanoparticle-assembly: fabrication of dynamic pH-responsive containers. Chemical Communications 48:856-858. 2012
Immune modulatory responses of Mesenchymal stem cells from different sources in cultures and in vivo. Cell & Tissue Transplantation & Therapy 4 :1-13 2012
Larval morphology and ontogeny of Nasikabatrachus sahyadrensis Biju & Bossuyt, 2003 (Anura, Nasikabatrachidae) from Western Ghats, India. Zootaxa 3510: 1-40 2012
Engineered ZnO and TiO2 nanoparticles induce oxidative stress and DNA damage leading to reduced viability of Escherichia coli. Free Radical Biology & Medicine 51:1872-1881 2011
Evaluation of Nano-Biphasic Calcium Phosphate Ceramics for Bone Tissue Engineering Application: In vitro and Preliminary In vivo studies" J Biomaterial Applications ( in press) 2011
NanoBiphasic Ceramics For Tissue Engineering: A Review. Journal of Biomedical Nanotechnology Dec Issue ( in press) 2011
A flow cytometric method to assess nanoparticle uptake in bacteria. CytometryA DOI: 10.1002/cyto.a.21085 2011
Cells Behaviour in Presence of Nano-Scaffolds. Journal of Biomedical Nanotechnology, 7(1) 43-44 2011
Repercussions of Mitochondria deformity Induced by anti-HSP90 Drug 17 AAG Human tumor cells. Drug Target Insights 2011:5 11-32 2011
Mitochondrial dysfunction and genetic heterogeneity in chronic periodontitis. Mitochondrion 11: (3) 504-512[4.5] 2011
Cellular uptake and mutagenic potential of metal oxide nanoparticles in bacterial cells. Chemosphere 83: 1124-1132 2011
ROS-mediated genotoxicity induced by titanium dioxide nanoparticles in human epidermal cells. Toxicology in Vitro 25:231-241 2011
Cellular permeation with nuclear infiltration capability of biomimetically synthesised fluorescent monodisperse mesoporous silica nanospheres in HeLa and human stem cells J Mat Chem. 20, 8563-8570 2010
Bio-inspired Silicification of Functional Materials: Fluorescent Monodisperse Mesostructure Silica Nanospheres Materials Chem. Mater., 2010, 22 (2), pp 551-556 2010
A novel nucleoid associated protein of M. tuberculosis is a sequence homolog of GroEL NAR 37:4944-54 2009
Mesenchymal cell response to nanosized biphasic calcium phosphate composites. Colloids and Surfaces B: Biointerfaces 73 (2009) 146-151 2009
Designed multi-domain protein as a carrier of nucleic acids into cells. Journal of Controlled Release 133 (2009) 154-160 2009
Organic solvent mediated self-association of an amyloid forming peptide from beta2-micro-globulin: An atomic force microscopy study. Peptide Science 90:783-91 2008
Morphology controlled assembly of ZnO nanostructures: A bioinspired method and visible luminescence. Chemistry European Journal 14; 6421-6427 2008
Silver on PEG-PU-TiO2 Polymer Nanocomposite Films: An Excellent System for Antibacterial Applications. Chem Mater; 20 ; 2455-2460 2008
A mouse model for Luminal epithelial like ER positive subtype of human breast cancer. BMC cancer, 7:180 2007
Photoreduction of Silver on bare & colloidal TiO2 nanoparticles/ nanotubes: Synthesis, Characterization and tested for Antibacterial outcome. J. Phys. Chem. C, 111, 13393-13397 2007
Cloning And Expression Of Human Islet Amyloid Polypeptide In Cultured Cells. Biochemical and Biophysical Research Communications 356 (2007) 622-628 2007
Antibacterial activity of linear peptides spanning the carboxy-terminal-sheet domain of arthropod defensins. Peptides 27:2614-2623 2006
Antibacterial activities of synthetic peptides corresponding to the carboxy terminal region of human defensin 1-3 Peptides 27:2607-2613 2006
Studies on the glycoprotein modification in Erythrocyte membrane during experimental cerebral malaria. Experimental Parasitol.114:173-179 2006
Clinical outcome of autologous cultivated limbal epithelium transplantation. Indian J Ophthalmol 54:29-34 2006
k_-Casein-deficient mice fail to lactate Proc. Nat Sci Acad USA 103:8000-8005 2006
Intranasal Mucoadhesive Microemulsions of Clonazepam: Preliminary Studies on Brain Targeting. Journal of Pharmaceutical Sciences 95: E1-E11 2006
Preliminary Brain-targeting Studies on Intranasal Mucoadhesive Micro-emulsions Sumatriptan. AAPS PharmSciTech. 6 :E1-E9 2006
A comparative study of epiretinal membranes associated with Eales` disease: A clinicopathologic evaluation Eye 20 (1): 46-54 2006
Protective effect of Spirulina against doxorubicin-induced cardiotoxicity. Phytothrapy Res. 19:1030-37 2005
Mechanism of thioflavin T binding to amyloid fibrils J Structural Biology 151: 229-238 2005
Is microsporidial keratitis an emerging cause of stromal keratitis? - a case series study BMC Ophthalmology 5:19 2005
Nanoparticle-Embedded Polymer: In Situ Synthesis, Free-Standing Films with Highly Monodisperse Silver Nanoparticles and Optical Limiting Chem Mater.17, 9-12 2005
Polygonal gold nanoplates in a polymer matrix Chem Comm :2387-2389 2005
Early results of penetrating keratoplasty after cultivated limbal epithelium transplantation. Arch Ophthalmol.; 123: 334-40 2005
Ex-vivo potential of cadaveric and fresh limbal tissues to regenerate cultured epithelium Indian J Ophthalmol 52:113-20. 2004
Variation of mitochondrial size during the cell cycle: A multiparameter flow cytometric and microscopic study Cytometry 62A:97-108 2004
Successful Reconstruction of damaged ocular outer surface in humans using limbal and conjunctival stem cell culture methods. Biosciences report 23:169-174 2003
Single Disulfide and Linear Analogues Corresponding to the Carboxy-Terminal Segment of Bovine -Defensin-2: Effects of Introducing the -Hairpin Nucleating Sequence D-Pro-Gly on Antibacterial Activity and Biophysical Properties. Biochemistry 42: 9307 - 9315 2003
Induction of autophagic cell death in Leishmania donovani by antimicrobial peptides. Molecular and Biochemical Parasitology 127:23-35 2003
Protective effects of Gingko biloba against doxorubucin-induced cardiotoxicity in mice. Ind J Exp. Biol. 40: 894-900.9 2002
Subepithelial amyloid deposits in Congenital Hereditary endothelial dystrophies- a histopathological study of 5 cases. Cornea.21:524-529 2002
Conventional type of Estrogen receptor a is found in the plasma membrane of Vaginal epithelial cells of Rat. Steroids 67:757-764 2002
Structure-Function Relationship on the Frog Skin Anti-microbial Peptide Tigerenin 1: Design of analogs with Improved acitivity and their action on Clinical Bacterial Isolates. Antimicrobial Agents and Chemotherapy 46: 2279-83 2002
Does Silicon oil peneterate lens capsules. Retina the Journal of Retinal and Vitreous diseases 21:275-277 2001
Deregulation of intermediate filament protein phosphorylation leads to pathogenesis: a review. Biomed. Letters 59:27-32 1999
Induction of Phospho inositide mediated signal transduction pathway by 17b estradiol in rat vaginal epithelial cells. J Mol. Endocrinol. 19: 249-257 1997
Morphological and Biochemical studies on Intracellular crystals : a review Cytobios 82; 101-122 1995
Tampering with cytokeratin expression leads to cell dysfunction. Epithelial Cell Biol 3:79-83 1994
Intermediate filaments- Heterogenous expression pattern and Modulations: Can their role in structure and function be ascertained Biol. Cell 82:1-10 1994
Multiple roles of Intermediate filaments Cytobios 77: 41-57 1994
Keratinization of rat vaginal epithelium V Modulations of intracellular calcium by estradiol. J Steroid Biochem. Mol. Biol.37:521-527 1990
Mineral concretions in Stylonychia mytilus Ehrenberg (Cliliata: Protozoa) Ehrenberg. Ind J Expt. Biol. 26:407-412 1987
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Shashi Singh

Chief Scientist

2934

shashis@ccmb.res.in

Vijayishwer Singh Jamwal

Project Assistant -II