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Swasti Raychaudhuri

Ramalingaswami Fellow (DBT)

Research Interests

Toxic and beneficial outcome of protein aggregation inside cells

With the increasing life expectancy, developing effective treatments for numerous degenerative, age-related diseases becomes of enormous medical, social and economic relevance. Hence, research on cellular and organismal aging is now the focus of several leading laboratories and has provided different hypotheses about the causes of aging. One prominent hypothesis suggests that a gradual, age-dependent decline of protein homeostasis (proteostasis) accompanied by increasing protein-aggregation is the underlying cause of many age-related loss-of-function diseases. In our lab we perform systemic analysis of protein-aggregation in age-related proteostasis stress models. We are interested to identify the response of the cellular proteome against the newly triggered aggregates due to various stresses. Simultaneously, we investigate how components of the proteostasis network, including molecular chaperones, degradation machinery and others collaborate to maintain the integrity of the proteome in the face of protein-aggregation stresses. The specific questions include:

  • Proteostatic control on protein-complex biogenesis!!
  • Cellular response to protein aggregation!!

We use modern cell biology and state-of-the-art proteomics arsenal to address our questions.

Selected Publications

Aggregation of Respiratory Complex Subunits Marks the Onset of Proteotoxicity in Proteasome Inhibited Cells. Rawat S, Anusha V, Jha M, Sreedurgalakshmi K, Raychaudhuri S. J Mol Biol. 2019 431(5):996-1015. 

Interplay of Acetyltransferase EP300 and the Proteasome System in Regulating Heat Shock Transcription Factor 1. Raychaudhuri S, Loew C, Koerner R, Pinkert S, Theis M, Hayer-Hartl M, Buchholz F, Hartl FU. Cell. 2014 Feb 27;156(5):975-85. 

Firefly luciferase mutants as sensors of proteome stress.Gupta R, Kasturi P, Bracher A, Loew C, Zheng M, Villella A, Garza D, Hartl FU, Raychaudhuri S.Nature Methods. 2011 Sep 4; 8(10):879-84. 

The role of intrinsically unstructured proteins in neurodegenerative diseases.Raychaudhuri S, Dey S, Bhattacharyya NP, Mukhopadhyay D. PLoS One. 2009;4(5):e5566. 

HYPK, a Huntingtin interacting protein, reduces aggregates and apoptosis induced by N-terminal Huntingtin with 40 glutamines in Neuro2a cells and exhibits chaperone-like activity.Raychaudhuri S, Sinha M, Mukhopadhyay D, Bhattacharyya NP. Hum Mol Genet. 2008 Jan 15;17(2):240-55. 

Education & Experiance

P.G: Biotechnology ; University of Calcutta ; 2002
Ph.D: Biophysical and Biological characterization of Huntingtin Interacting Protein HYPK ; University of Calcutta ; 2008
Post.Doc:

Protein misfolding, Stress response ; Max Planck Institute for Biochemistry ; 2008-2013

Publications

TitleJournalYear
Aggregation of Respiratory Complex Subunits Marks the Onset of Proteotoxicity in Proteasome Inhibited Cells J Mol Biol 2019
Cytoplasmic sequestration of the RhoA effector mDiaphanous1 by Prohibitin2 promotes muscle differentiation. Scientific Reports 2019
Identification of a splice variant of optineurin which is defective in autophagy and phosphorylation Biochim Biophys Acta Mol Cell Res. 2018
Interplay of Acetyltransferase EP300 and the Proteasome System in Regulating Heat Shock Transcription Factor 1 Cell 2014
Conserved C-terminal nascent peptide binding domain of HYPK facilitates its chaperone-like activity Journal of Biosciences 2014
Firefly luciferase mutants as sensors of proteome stress Nature Methods 2011
Protein Folding in the Cytoplasm and the Heat Shock Response Cold Spring Harb Perspect Biol 2010
Identification of HYPK interacting proteins reveals involvement of HYPK in regulating cell growth, cell cycle, unfolded protein response and cell death PLoS One 2009
HYPK, a Huntingtin interacting protein, reduces aggregates and apoptosis induced by N-terminal Huntingtin with 40 glutamines in Neuro2a cells and exhibits chaperone-like activity Hum Mol Genet 2008
Huntingtin interacting protein HYPK is intrinsically unstructured Proteins 2008
Bacterial exotoxins downregulate cathelicidin (hCAP-18/LL-37) and human beta-defensin 1 (HBD-1) expression in the intestinal epithelial cells Cell Microbiol 2008
Increased caspase-2, calpain activations and decreased mitochondrial complex II activity in cells expressing exogenous huntingtin exon 1 containing CAG repeat in the pathogenic range Cell Mol Neurobiol 2007

Swasti Raychaudhuri

Ramalingaswami Fellow (DBT)

2558

rcswasti@ccmb.res.in

Shivali Rawat

Senior Research Fellow

2551

shivali@ccmb.res.in

Shemin Mansuri

Senior Research Fellow

2551

shemin.16@ccmb.res.in

Harshit Vaish

Junior Research Fellow

2551

harshit@ccmb.res.in

Pooja Singh

Junior Research Fellow

2551

poojagupta@ccmb.res.in

Suparna Ghosh

Junior Research Fellow (Project)

2551

suparna@ccmb.res.in

Richa Singh

Junior Research Fellow (Project)

2551

richa@ccmb.res.in