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Research Interests

The research activities of the laboratory center around the understanding of diverse group of calcium-binding proteins with emphasis on the functional and structural requirement of calcium-binding. The families we are concentrating are neuronal calcium sensors (EF-hand superfamily) and bg-crystallin superfamily.

Selected Publications

Srivastava,S.S., Mishra, A., Krishnan, B., Sharma, Y. (2014) ca2+-binding motif of ??-crystallins. J. Biol. Chem. 289(16), 10958-10966. (Minireview)

Mishra, A., Krishnan, B., Srivastava, S.S., Sharma, Y. (2014) Microbial ??-crystallins. Progress in Biophysics and Molecular Biology 115(1), 42-51. (review)

Mishra, A., Suman, S.K., Srivastava, S.S., Sankaranarayanan, R. Sharma, Y., (2012) Decoding the molecular design principles underlying Ca2+ -binding to ??-crystallin motifs. J. Mol. Biol. 415, 75-91.

Suman, S. K., Mishra, A., Ravindra, D., Yeramala, L., Sharma, Y. (2011) Evolutionary remodelling of the ??-crystallins for domain stability at the cost of Ca2+ -binding. J. Biol. Chem. 286, 43891-43901.

Mikhaylova, M., Reddy, P. P., Munsch, T., Landgraf, P. Suman, S. K., Smalla, K.H., Gundelfinger, E. D., Sharma, Y., Kreutz, M. R. (2009) Calneurons provide a Calcium-threshold for trans-Golgi network to plasma membrane trafficking. Proc. Natl. Acad. Sci. USA 106, 9093-9098. (Selected for the Faculty of 1000 Biology)

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Publications

TitleJournalYear
Interface interactions between ??-crystallin domain and Ig-like domain render Ca2+-binding site inoperative in abundant perithecial protein of Neurospora crassa. Molecular Microbiology (2018)0(0), 1-18 2018
Strategizing for the purification of a multiple Big domain-containing protein in native conformation is worth it! Protein Expr Purif. 2018 May;145:25-31. doi: 10.1016/j.pep.2017.12.008. Epub 2017 Dec 27. 2018
A transition metal-binding, trimeric ??-crystallin from methane producing thermophilic archaea Methanosaeta thermophila. Biochemistry 56, 1299-1310.(featured on journal cover page) 2017
Secretagogin is a redox-responsive Ca2+ sensor. Biochemistry 56, 411-420. 2017
Intermotif Communication Induces Hierarchical Ca2+Filling of Caldendrin. Biochemistry. 2017 May 16;56(19):2467-2476. 2017
A Transition Metal-Binding, Trimeric ??-Crystallin from Methane-Producing Thermophilic Archaea, Methanosaeta thermophila. Biochemistry. 2017 Mar 7;56(9):1299-1310 2017
1H, 13C and 15N NMR assignments of a bacterial immunoglobulin-like domain (group 2) of a protein of a bacterium Paenarthrobacter aurescens TC1. Biomol NMR Assign. 2017 Oct;11(2):203-206. doi: 10.1007/s12104-017-9748-5. Epub 2017 Jun 7. 2017
Ca2+ and ??-crystallins: An affair that did not last? Biochim Biophys Acta. 1860, 299-303. 2016
The Mycobacterium tuberculosis desaturase DesA1 (Rv0824c) is a Ca2+ binding protein. Biochem. Biophys. Res. Commun. 480(1), 29-35. 2016
The PE_PGRS proteins of Mycobacterium tuberculosis are Ca2+-binding mediators of host-pathogen interaction. Biochemistry 55(33), 4675-4687 2016
Optimization of purification method and characterization of recombinant human Centrin-1. Protein Expr Purif. 2016 Aug;124:48-54. doi: 10.1016/j.pep.2016.05.012. Epub 2016 May 24. 2016
Secretagogin, a hexa EF-hand calcium-binding protein: High level bacterial overexpression, one-step purification and properties. Protein Expr. Purif. 109C, 113-119 2015
Liaison between Myristoylation and Cryptic EF-Hand Motif Confers Ca2+ Sensitivity to Neuronal Calcium Sensor-1. Biochemistry 54(4): 1111-1122. 2015
Molecular Dynamics of the Neuronal EF-Hand Ca2+-Sensor Caldendrin PLoS One.0103186 2014
NMR Solution Structure of the Terminal Immunoglobulin-like Domain from the Leptospira Host-Interacting Outer Membrane Protein, LigB Biochemistry. 53(32):5249-5260 2014
Microbial ??-crystallins. Progress in Biophysics and Molecular Biol. 115(1), 42-51 2014
Ca2+-binding motif of ??-crystallins J. Biol. Chem. 289(16), 10958-10966. 2014
?-Carotene attenuates angiotensin II-induced aortic aneurysm by alleviating macrophage recruitment in Apoe-/- mice PLoS One. 8(6), e67098 2013
Association properties and unfolding of a ??-crystallin domain of a Vibrio-specific protein PLoS One. 8(1): e53610 2013
Perturbation of tryptophan fluorescence by phenylalanine to alanine mutations identifies the hydrophobic core in a subset of bacterial ig-like domains Biochemistry.52, 4589-4591 2013
Disability for function: loss of Ca2+-binding is obligatory for fitness of mammalian ??-crystallins Biochemistry. 52,9047-9058 2013
Decoding the molecular design principles underlying Ca2+-binding to ??-crystallin motifs. J. Mol. Biol. 415, 75-91. 2012
Ca(2+) sensor proteins in dendritic spines: a race for Ca(2+) Front Mol Neurosci. 5, 61. 2012
Aggregation-prone near-native intermediate formation during unfolding of a structurally similar nonlenticular ??-crystallin domain Biochemistry. 51, 8502-8513. 2012
N-terminal myristoylation alters the calcium binding pathways in neuronal calcium sensor-1. J Biol Inorg Chem. 16(1), 81-95. 2011
Temperature-dependent oligomerization in M-crystallin: Lead or lag toward cataract, an NMR perspective. Proteins. 79(2):569-580. 2011
Structural basis for preferential Ca2+-displacement by a paramagnetic ion (Ce3+) in non-myristoylated neuronal calcium sensor-1. Ind. J. Chem. 50A, 539-547. 2011
Characterization of Low Energy Excited States in the Native State Ensemble of Non-myristoylated Neuronal Calcium Sensor-1. BBA Section: BBA - Proteins and Proteomics 1814(2), 334-344. 2011
Leptospira Immunoglobulin-like protein (LigB) binds to the C-terminal fibrinogen C domain inhibiting fibrin clot formation, platelet adhesion and aggregation. Mol. Microbiol. 79(4):1063-1076. 2011
Posttranslational membrane insertion of the tail anchored transmembrane EF-hand Ca2+-sensors Calneurons requires the TRC40/Asna1 chaperone. J. Biol. Chem. 286, 36762-36776. 2011
Evolutionary remodelling of the betagamma-crystallins for domain stability at the cost of Ca2+-binding. J. Biol. Chem. 286, 43891-43901. 2011
A Natively Unfolded ??-Crystallin Domain from Hahella chejuensis. Biochemistry 49(45), 9746-9755. 2010
(2010) Big Domains are Novel Ca2+-binding Modules: Evidences from Big Domains of Leptospira immunoglobulin-like (Lig) Proteins. PLoS One 5(12), e14377. 2010
Comparative proteomic analysis of differentially expressed proteins in primary retinoblastoma tumors. Proteomics Clin. Appl. 4 (4), 449-463. 2010
Resonance assignments of myristoylated and non-myristoylated neuronal calcium sensor-1 (NCS-1) embedded in a membrane. Biomol NMR Assign. 2010 May 20. 2010
The terminal immunoglobulin-like repeats of LigA and LigB of Leptospira enhance their binding to gelatin binding domain of fibronectin and host cells. PLoS One. 5(6), e11301. 2010
Complete backbone assignment of a Ca2+-binding protein of the betagamma-crystallin superfamily from Methanosarcina acetivorans, at two denaturant concentrations. J. Biomol NMR Assign , (DOI 10.1007/s12104-009-9152-x). 2009
Three-dimensional domain swapping in nitrollin, a single-domain beta gamma-crystallin from Nitrosospira multiformis, controls protein conformation and stability but not dimerization. J. Mol. Biol., 385: 163-177. 2009
Solution structure and calcium-binding properties of M-crystallin, a primordial betagamma-crystallin from archaea. J. Mol. Biol., 386: 675-689 2009
Structural differences between the SH3-HOOK-GuK domains of SAP90/PSD-95 and SAP97. Protein Expr Purif. 68(2), 201-207. 2009
Repeated Domains of Leptospira Immunoglobulin-like Proteins Interact with Elastin and Tropoelastin. J. Biol. Chem. 284(29), 19380-19391. 2009
A novel fibronectin type III modules binding motif identified on C-terminus of Leptospira Immunoglobulin-like protein LigB. Biochem. Biophys. Res. Commun. 389, 57-62. 2009
Mikhaylova, M., Reddy, P. P., Munsch, T., Calneurons provide a Calcium-threshold for trans-Golgi network to plasma membrane trafficking. Proc. Natl. Acad. Sci. USA 106(22), 9093-9098. (Selected for the Faculty of 1000 Biology) 2009
Fibronectin binds to and induces conformational change in a disordered region of Leptospira interrogans immunoglobulin-like protein LigB. J. Biol. Chem. 284, 23547-23557. 2009
The betagamma-crystallin superfamily contains a universal motif for binding calcium. Biochemistry 48, 12180 2009
Overexpression, on-column refolding adn isotopic labeling of Hahellin from Hahella Chejuensis, a putative member of the beta-gamma-crystallin superfamily Protein Expre. Purif., 58: 269-274 2008
Regulatory and structural EF-hand motifs of neuronal calcium sensor-1 Mg2+ modulates Ca2+ -binding, Ca2+-induced conformation adn equilibrium unfolding transitions J. Mol. Biol., 376: 1100-1115 2008
Exploring the limits of sequence and structure in a variant beta gamma-crystallin domain of AIMI J. Mol. Biol., 381: 509-518 2008
Calcium binds to Leptospiral immunoglobulin-like protein, LigB and modulates fibronectin-binding J. Biol. Chem., 283(37): 25140-25149 2008
Calcium-binding to lens betaB2- and betaA3-crystallins suggests that all beta-crystallins are calcium-binding proteins. FEBS J. 274; 4135-4147. 2007
Caulollins from Caulobacter crescentus, a pair of partially unstructured proteins of betagamma-crystallin superfamily gains structure upon binding calcium. Biochemistry 46, 12298-12307. 2007
Ubiquitous lens alpha-, beta- and gamma-crystallins accumulate in anuran cornea as corneal crystallins. J. Biol. Chem. 282, 18953-18959. 2007

Yogendra Sharma

J C Bose Fellow

2561

yogendra@ccmb.res.in

Syed Sayeed Abdul

Lab Attendant(2)

2562

sayeed@ccmb.res.in

Venu Sankeshi

SERB National Post-Doctoral Fellow

2562

venu.sankeshi@ccmb.res.in

Anand Kumar Sharma

Senior Research Fellow

2562

anandkumar@ccmb.res.in

Asmita Dhansing Pawar

Senior Research Fellow(UGC)

2562

pawarsmita@ccmb.res.in

Radhika Khandelwal

Senior Research Fellow

2562

radhika.khandelwal@ccmb.res.in

Aditya A Jamkhindikar

Senior Project Fellow

2562

aditya.jamkhindikar@ccmb.res.in

Amrutha H C

Senior Research Fellow(ICMR)

2559

amruthahc@ccmb.res.in

P Sai Uday Kiran

Junior Research Fellow(UGC)

2562