Reference Panels for identification and individualization of Station-bred Selections and Rust Differentials

 

DNA typing of selected elite Coffee genotypes was undertaken using multiple DNA marker techniques like RAPD, Flourescence-AFLP and Flourescence-microsatellites (SSRs) for their genetic characterisation and also to create genotype-specific diagnostic patterns. The DNA profiles, thus obtained, were analyzed to develop Reference DNA Polymorphism data panels that can be used readily to generate "Molecular ID's" unique to specific coffee genotypes. Efforts were made to identify sets of microsatellite markers (which provide more robust easily revisitable signatures compared to RAPDs/AFLPs etc.) and that were relatively more polymorphic for easy individualization of CCRI-bred Arabica Selections and rust differentials. Importantly, a set of about 6-10 microsatellite markers of the 80-100 total tested ones could be identified that were capable of completely distinguishing all the Station-bred Selections and Rust differentials individually. Such reference polymorphism panels are expected to be of immense use for genotype registration, IPR protection and for other genetic studies.

We now have such panels created for selected arabica selections, coffee rust differentials, elite parental genotypes (used in various crossing programs) and some of the related taxa of coffee, using a number of decamer RAPD primers, 10 AFLP primer combinations (each primer being 3-base selection), about 100 in-house developed coffee specific microsatellite markers.

Two of the representative Polymorphism Data Panels for which some of the microsatellite markers are shown below. Information /data for other markers can be obtained from CCMB on specific requests on the contact address given below.

 

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For further details Contact :

Dr. Ramesh K. Aggarwal

Scientist E II / Project Leader
Centre for Cellular and Molecular Biology (CCMB) Hyderabad-500007, India.
Phone: +91-040-27192635 Fax +91-040-27160591 /27160311
email: rameshka@ccmb.res.in

 

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