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Facilities
Laboratory
Animal
Animal
House
The Animal House Facility maintains thirty one strains of mice (inbred, outbred, transgenic, knock-out & immune deficient ) , three strains of rats, two strains of hamsters, three strains of rabbits and two strains of guinea pigs in different rooms. The average number of animals at any one time is about 6000. The animal rooms are maintained at
22.2ΊC with a relative humidity of 55.5%. A 12-hour light-dark cycle is maintained with automatic timers. All the environmental factors are moderated by a computerized central monitoring system. The animal facility is registered under CPCSEA, Government of India.
The main objective of the Animal House is to supply defined strains of mice, rats, guinea pigs, hamsters and rabbits to the scientific community of the Centre. The propagation of all defined strains is done in two-tier system, i.e., foundation colony (SPF colony) and production colony.
Mouse genotype laboratory was recently established with equipment related to genetic monitoring and biochemical monitoring of laboratory animals. Routine check for genetic contamination in lab animals is carried out using nine microsatellite SSLP markers (D1 Mit 21,D4 Mit 53, D5 Mit 18, D4 Mit 7, D15 Mit 8, D16 Mit 4, D14 Mit 40, D19 Mit 41 and D2Mit61) and ten biochemical markers ( Hbb, Car- I, Car- II, Gpi- I, Est- I, Est- III, Mod- I, Idh- I, Pgm- I and Trf- I ) using PCR and enzyme markers analyzed through cellulose acetate electrophoresis techniques.
The Animal House team comprises of 18 staff including 11 qualified animal caretakers, who have undergone specialized training in breeding and management of laboratory animals course offered by the Indian Council of Medical Research at the Laboratory Animal Information Service Centre, Hyderabad.
A trained veterinarian carries out regular health monitoring of all animals to ensure that they are free from contagious diseases. To prevent the transmission of infection, standard hygienic procedures, i.e., use of sterilized bedding, autoclaved cages, zero
bacteria/autoclaved drinking water and handling of animals using gloved hands are followed. Necropsy and autopsy of suspected contagious/infectious animals are performed by a veterinarian and prophylactic measures are implemented.
Regular screening of microorganisms in the SPF and production colony mice, feed, water, paddy husk, and isolators are carried out by various microbiological and pathological techniques, viz., culture, differential staining techniques, biochemical tests and histopathology. All animal house records are updated and maintained everyday, assisted by unique software.
Mice
strains available
Mice:
In bred strains
| FVB/NJ |
C57
BL / 6 J |
CBA/
CaJ |
C3H
/ HeJ |
| Balb
C/J |
Neo
r |
A
129 J |
We
+ - mutant mice |
| R
III mutant mice |
Y
Del mutant mice |
SXR
mutant mice |
YMET
mutant mice |
| DBA/
2J |
|
|
|
Out
bred strains
1 . CD 1
Transgenic/
knockout mice (Disease models)
| C57
BL / 6 J Tg N HBV Hepatitis B |
C57
BL / 6 J Apob - Atherosclerosis |
C57
BL / 6J Apo e Alzheimers disease |
AkR/
J _ Leukemia |
| NOD
/ Lt J - Diabetes |
Kcas
knock out mice (milk production gene)* |
WDR
Transgenic & knockout mice* |
MDX
Muscular dystrophy mice |
| MMTV
cre mammary tumor animal model |
Wap
Cre mammary tumor animal model |
BRCA
1 knockout- mammary tumor animal model |
Db/db
diabetes animal model |
| B6.129X1-GPd2tm1Lbr/Mmcd
animal model for severe hypoglycemia,
ketosis, and glyceroluria |
B6C3TG
(APPSWE)pSEN1DE 85 DBO/J - Parkinson
disease model |
|
|
*CCMB
produced these mice
Immunodeficient
mice
| Scid
Mice (Prkdc - Balb / c background) |
Nude
mice (Fox N1 strain ) |
NIH
nude mice |
Beige
mice( NK cells deficient ) |
Rats
| Wistar
Rats |
CFY
rat |
Sprague
Dawley |
Rabbit
| New
Zealand white |
Grey
Giant |
Flemish
Giant |
Hamster
| Syrian
Hamster |
NIN
hamster |
Guinea
Pig
|
